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Effect of heat stress on production and in-vitro antioxidant activity of polysaccharides in Ganoderma lucidum

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Abstract

Ganoderma lucidum is a traditional Chinese medicine, and its polysaccharides possess diverse and significant pharmacological activities. This study aimed to investigate the polysaccharide production, molecular characteristics and in-vitro antioxidant activity of G. lucidum fruiting body after the mushroom was harvested and treated with heat stress (HS). HS enhanced the production of polysaccharides after harvest and treatment of 42 °C HS for 2 h, and that resulted in the highest polysaccharide yield of 10.50%, which was 45.63% higher than that of the control, while 37, 45 °C HS had no significant effect on the production. In terms of molecular characteristics, 42 °C HS significantly changed monosaccharide ratio of polysaccharides, but no apparent molecular weight and functional group changes were found in polysaccharides after HS treatment. The results of in-vitro antioxidant activity assay revealed that 42 °C HS significantly improved the antioxidant activities of polysaccharides at the concentration of 2 mg/mL. In conclusion, this study provides a promising strategy to improve the production of G. lucidum fruiting body polysaccharides.

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Acknowledgements

This work was supported by the Chinese Universities Scientific Fund (Grant no. 2017SP001); and National Natural Science Foundation of China (Grant no. 21576142).

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Authors and Affiliations

  1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, Beijing, 100083, People’s Republic of China

    Xiaoyan Tan, Hengchang Li, Jing Hu, Huijuan Ning, Zifang Qin & Xiuqing Zhang

  2. Chinese Academy of Agricultural Engineering, Beijing, 100125, People’s Republic of China

    Junshe Sun & Haisheng Pei

  3. Department of Biological Systems Engineering, Washington State University, Richland, WA, USA

    Zhangyang Xu

  4. Tianfangjian (China) Pharma Company Ltd, Guangzhou, 510623, People’s Republic of China

    Tian Sun

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  1. Xiaoyan Tan
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  2. Junshe Sun
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  4. Hengchang Li
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  5. Jing Hu
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  9. Tian Sun
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Correspondence to Xiuqing Zhang.

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449_2017_1850_MOESM1_ESM.tif

Fig. S1 HPGPC chromatograms of fruiting body polysaccharides without 42 °C HS (FCK) and with 42 °C HS (FHS). The peaks without numbering were those beyond the separation range or cannot be identified aspolysaccharides (less than 1800 Da) (TIF 609 KB)

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Tan, X., Sun, J., Xu, Z. et al. Effect of heat stress on production and in-vitro antioxidant activity of polysaccharides in Ganoderma lucidum . Bioprocess Biosyst Eng 41, 135–141 (2018). https://doi.org/10.1007/s00449-017-1850-7

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  • DOI: https://doi.org/10.1007/s00449-017-1850-7

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