Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4

Bioprocess and Biosystems Engineering volume 40, pages395–402(2017)Cite this article

Abstract

Saline waste water from demineralization of cheese whey was used as the main component of waste saline medium (WSM) for cultivation of thraustochytrids. The suitability of WSM for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4 was evaluated by comparison with cultivation on nutrient medium (NM) in shake flask and fermenter cultures. Biomass productivities achieved in WSM for the thraustochytrids were comparable with those in NM for both shake flask and fermenter cultures. The maximum total lipid content (56.71% dry cell weight) and docosahexaenoic acid productivity (0.86 g/L/day) were achieved by J. marinum AN-4 grown on WSM in shake flask and fermenter cultures, respectively. A cost estimate of WSM suggests that this medium could result in lower production costs for thraustochytrid biomass and lipids and contribute to the effective reduction in saline diary process waste water.

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Acknowledgements

This work was supported by the Technology Agency of the Czech Republic through project TE01020080-BIORAF.

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Affiliations

  1. Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28, Prague, Czech Republic

    Tomas Humhal, Petr Kastanek, Zuzana Jezkova, Anna Cadkova & Tomas Branyik

  2. EcoFuel Laboratories Ltd., Ocelarska 392/9, 190 00, Prague, Czech Republic

    Petr Kastanek

  3. Department of Food Analysis and Nutrition, University of Chemistry and Technology Prague, Technicka 5, 166 28, Prague, Czech Republic

    Jana Kohoutkova

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  1. Tomas Humhal
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Correspondence to Tomas Branyik.

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Humhal, T., Kastanek, P., Jezkova, Z. et al. Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4. Bioprocess Biosyst Eng 40, 395–402 (2017). https://doi.org/10.1007/s00449-016-1707-5

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Keywords

  • Saline waste water
  • Schizochytrium limacinum
  • Japonochytrium marinum
  • Docosahexaenoic acid
  • Shake flask
  • Bioreactor