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Bioconversion of waste office paper to hydrogen using pretreated rumen fluid inoculum

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Abstract

In this study, a microbial consortium from an acid-treated rumen fluid was used to improve the yields of H2 production from paper residues in batch reactors. The anaerobic batch reactors, which contained paper and cellulose, were operated under three conditions: (1) 0.5 g paper/L, (2) 2 g paper/L, and (3) 4 g paper/L. Cellulase was added to promote the hydrolysis of paper to soluble sugars. The H2 yields were 5.51, 4.65, and 3.96 mmol H2/g COD, respectively, with substrate degradation ranging from 56 to 65.4 %. Butyric acid was the primary soluble metabolite in the three reactors, but pronounced solventogenesis was detected in the reactors incubated with increased paper concentrations (2.0 and 4.0 g/L). A substantial prevalence of Clostridium acetobutylicum (99 % similarity) was observed in the acid-treated rumen fluid, which has been recognized as an efficient H2-producing strain in addition to ethanol and n-butanol which were also detected in the reactors.

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Acknowledgments

The authors gratefully acknowledge the financial support of CNPq—National Council for Scientific and Technological Development and FAPESP—São Paulo Research Foundation.

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Authors and Affiliations

  1. Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100, Jd. Santa Angelina, 13563-120, São Carlos, SP, Brazil

    Lívia Silva Botta, Regiane Priscila Ratti, Isabel Kimiko Sakamoto & Maria Bernadete Amâncio Varesche

  2. Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, Km 235, 13565-905, São Carlos, SP, Brazil

    Lucas Rodrigues Ramos & Edson Luiz Silva

Authors
  1. Lívia Silva Botta
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  2. Regiane Priscila Ratti
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  3. Isabel Kimiko Sakamoto
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  4. Lucas Rodrigues Ramos
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  5. Edson Luiz Silva
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  6. Maria Bernadete Amâncio Varesche
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Correspondence to Edson Luiz Silva.

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Botta, L.S., Ratti, R.P., Sakamoto, I.K. et al. Bioconversion of waste office paper to hydrogen using pretreated rumen fluid inoculum. Bioprocess Biosyst Eng 39, 1887–1897 (2016). https://doi.org/10.1007/s00449-016-1663-0

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  • DOI: https://doi.org/10.1007/s00449-016-1663-0

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