The nanofibrous PAN-PANi scaffold as an efficient substrate for skeletal muscle differentiation using satellite cells
- 417 Downloads
Among polymers, polyaniline (PANi) has been introduced as a good candidate for muscle regeneration due to high conductivity and also biocompatibility. Herein, for the first time, we report the use of electrospun nanofibrous membrane of PAN-PANi as efficient scaffold for muscle regeneration. The prepared PAN-PANi electrospun nanofibrous membrane was characterized by scanning electron microscopy (SEM), Attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR) and tensile examination. The softer scaffolds of non-composite electrospun nanofibrous PAN govern a higher rate of cell growth in spite of lower differentiation value. On the other hand, PAN-PANi electrospun nanofibrous membrane exposed high cell proliferation and also differentiation value. Thank to the conductive property and higher Young’s modulus of composite type due to the employment of PANi, satellite cells were induced into more matured form as analyzed by Real-Time PCR. On the other hand, grafting of composite nanofibrous electrospun scaffold with gelatin increased the surface stiffness directing satellite cells into lower cell proliferation and highest value of differentiation. Our results for first time showed the significant role of combination between conductivity, mechanical property and surface modification of PAN-PANi electrospun nanofibers and provid new insights into most biocompatible scaffolds for muscle tissue engineering.
KeywordsPolyaniline Nanofibers Conductive scaffolds Surface stiffness Satellite cells
This work was supported by a Grant of Stem Cell Technology Research Center, Tehran, Iran.
- 7.Ravichandran R, Sundarrajan S, Venugopal JR, Mukherjee S, Ramakrishna S (2010) Applications of conducting polymers and their issues in biomedical engineering. J R Soc Interface (rsif20100120) Google Scholar
- 20.Veluru JB, Satheesh K, Trivedi D, Ramakrishna MV, Srinivasan NT (2007) Electrical properties of electrospun fibers of PANI-PMMA composites. J Eng Fibers Fabrics 2:25–31Google Scholar
- 36.Farsani RE, Raissi S, Shokuhfar A, Sedghi A (2009) FT-IR study of stabilized PAN fibers for fabrication of carbon fibers. World Acad Sci, Eng Technol 50:430–433Google Scholar
- 37.Khalid M, Tumelero MA, Brandt IS, Zoldan VC, Acuña JJ, Pasa AA (2013) Electrical conductivity studies of polyaniline nanotubes doped with different sulfonic acids. Indian J Mater SciGoogle Scholar