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Scale-up laccase production from Trametes versicolor stimulated by vanillic acid

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Abstract

An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old mycelia cultures to 80 mg/L vanillic acid. After 4 days, laccase activity of 588.84 U/L was achieved in flasks which represented a 1.79-fold increase compared to the control. In 200-L airlift bioreactor, the maximal laccase activity reached up to 785.12 U/L using the optimized vanillic acid treatment strategy. The zymograms of culture supernatants revealed three bands with laccase activity, among which Lac1 and Lac2 were abundant laccase isoforms constitutively expressed, and Lac3 was an inducible isozyme by vanillic acid. The results of real-time quantitative PCR showed that the transcription level of lcc in T. versicolor cultures grown with vanillic acid for 7 days was about 5.64-fold greater than that without vanillic acid in flasks. In 200-L airlift bioreactor cultures of T. versicolor with addition of vanillic acid, the transcript level of lcc at day 7 was 2.62-fold higher than that in flasks with vanillic acid due to the good mass transfer and oxygen supply in the bioreactor system. This study provides a basis for understanding the induction mechanism of vanillic acid for laccase production and has good potential for industrial applications.

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Acknowledgments

This work was financially supported by the National Basic Research Program (973 Program) of China (No. 2013CB733600), the National Natural Science Foundation of China (No. 21476242), the National Key Technology Research and Development Program of China (No. 2015BAK45B01), the National High Technology Research and Development Program (863 Program) of China (No. 2012AA101803), the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSZD -EW-Z-015). We acknowledge Dr. Lim Jit Kang (the Chinese Academy of Sciences Fellowships for Young International Scientists, No. 2015VMC060) for help in editing this manuscript.

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China

    Ke-Feng Wang, Jian-Hua Hu, Chen Guo & Chun-Zhao Liu

  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Ke-Feng Wang, Jian-Hua Hu & Chen Guo

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  1. Ke-Feng Wang
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  2. Jian-Hua Hu
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Correspondence to Chun-Zhao Liu.

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Wang, KF., Hu, JH., Guo, C. et al. Scale-up laccase production from Trametes versicolor stimulated by vanillic acid. Bioprocess Biosyst Eng 39, 1041–1049 (2016). https://doi.org/10.1007/s00449-016-1582-0

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