Abstract
A novel method was developed for facile immobilization of enzymes on silica surfaces. Herein, we describe a single-step strategy for generating of reactive double bonds capable of Michael addition on the surfaces of silica particles. This method was based on reactive thin film generation on the surfaces by heating of impregnated self-curable polymer, alpha-morpholine substituted poly(vinyl methyl ketone) p(VMK). The generated double bonds were demonstrated to be an efficient way for rapid incorporation of enzymes via Michael addition. Catalase was used as model enzyme in order to test the effect of immobilization methodology by the reactive film surface through Michael addition reaction. Finally, a plug flow type immobilized enzyme reactor was employed to estimate decomposition rate of hydrogen peroxide. The highly stable enzyme reactor could operate continuously for 120 h at 30 °C with only a loss of about 36 % of its initial activity.
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Brinkman RP, Kratzer M, Schmidt H (1999) Simulation of plasma processes for microelectronic fabrication. Pure Appl Chem 71:1863–1869
Yi S, Kim J-K, Yue CY, Hsieh J-H (2000) Bonding strengths at plastic encapsulant gold plated copper-lead frame interface. Microelectron Reliab 40:1207–1214
Suzuki Y (2003) Ion beam modification of polymers for the application of medical devices. Nucl Instrum Meth B 206:501–506
Liston EM, Martinu L, Wertheimer MR (1993) Plasma surface modification of polymers for improved adhesion: a critical review. Adhes Sci Techol 7:1091–1127
Grzegorzewski F, Rohn S, Quade A, Schroder K, Ehlbeck J, Schluter O, Kroh LW (2010) Reaction chemistry of 1,4-benzopyrone derivates in non-equilibrium low-temperature plasmas. Plasma Process Polym 7:466–473
Bicak N (2014) A facile method for generating Michael acceptor thin films via amine substituted poly(vinyl methyl ketone). Pure Appl Chem 86:1829–1838
Bayramoglu G, Arica MY (2010) Reversible immobilization of catalase on Fe(III) chelated poly(itaconic acid) grafted chitosan membranes. J Mol Catal B 62:297–304
Todero LM, Bassi JJ, Lage FAP, Corradini MCC, Barboza JCS, Hirata DB, Mendes AA (2015) Enzymatic synthesis of isoamyl butyrate catalyzed by immobilized lipase on poly-methacrylate particles: optimization, reusability and mass transfer studies. Bioprocess Biosyst Eng 38:1601–1613. doi:10.1007/s00449-015-1402-y
Bayramoglu G, Karagoz B, Yilmaz M, Bicak N, Arica MY (2011) Immobilization of catalase via adsorption on poly(styrene-d-glycidylmethacrylate) grafted and tetraethyldiethylene triamine ligand attached microbeads. Bioresour Technol 102:3653–3661
Demirbas O, Alkan M, Demirbas A (2013) Surface properties of catalase vand casein on kaolinite and design of experiments. Micropor Mesopor Mater 172:151–160
Bressani APP, Garcia KCA, Hirata DB, Mendes AA (2015) Production of alkyl esters from macaw palm oil by a sequential hydrolysis/esterification process using heterogeneous biocatalysts: optimization by response surface methodology. Bioprocess Biosyst Eng 38:287–297
Bayramoglu G, Arica MY (2012) Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor. Bioprocess Biosyst Eng 35:423–431
Mohajershojaei K, Mahmoodi NM, Khosravi A (2015) Immobilization of laccase enzyme onto titania nanoparticle and decolorization of dyes from single and binary systems. Biotechnol Bioprocess Eng 20:109–116
Preety Hooda V (2014) Immobilization and kinetics of catalase on calcium carbonate nanoparticles attached epoxy support. Appl Biochem Biotechnol 172:115–130
Feng Q, Hou D, Zhao Y, Xu T, Menkhaus TJ, Fong H (2014) Electrospun regenerated cellulose nanofibrous membranes surface-grafted with polymer chains/brushes via the atom transfer radical polymerization method for catalase immobilization. ACS Appl Mater Interfaces 6:20958–20967
Feng Q, Zhao Y, Wei A, Li C, Wei Q, Fong H (2014) Immobilization of catalase on electrospun PVA/PA6−Cu(II) nanofibrous membrane for the development of efficient and reusable enzyme membrane reactor. Environ Sci Technol 48:10390–10397
Cowana DA, Fernandez-Lafuente R (2011) Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization. Enzyme Microb Technol 49:326–346
Bayramoglu G, Arica MY (2014) Activity and stability of urease entrapped in thermo-sensitive p(isopropylacrylamide-co-p(ethyleneglycole)-methacrylate) hydrogel. Bioprocess Biosyst Eng 37:235–243
Bayramoglu G, Altintas B, Arica MY (2012) Cross-linking of horseradish peroxidase adsorbed on polycationic films: utilization for direct dye degradation. Bioprocess Biosyst Eng 35:1355–1365
Yang D, Wang X, Shi J, Wang X, Zhang S, Han P, Jiang Z (2016) In situ synthesized rGO–Fe3O4 nanocomposites as enzyme immobilization support for achieving high activity recovery and easy recycling. Biochem Eng J 105:273–280
Zang J, Jia S, Liu Y, Wu S, Zhang Y (2012) A facile method to prepare chemically cross-linked and efficient polyvinyl alcohol/chitosan beads for catalase immobilization. Catal Commun 27:73–77
Li Y, Wang X-Y, Jiang X-P, Ye J-J, Zhang Y-W, Zhang X-Y (2015) Fabrication of graphene oxide decorated with Fe3O4@SiO2 for immobilization of cellulose. J Nanopart Res 17:8
Cengiz S, Cavas L, Yurdakoc K (2012) Bentonite and sepiolite as supporting media: immobilization of catalase. Appl Clay Sci 65–66:114–120
Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Cetinus SA, Sahin E, Saraydin D (2009) Preparation of Cu(II) adsorbed chitosan beads for catalase immobilization. Food Chem 114:962–969
Chen N, Huang S, Liao XP, Shi B (2011) Immobilization of catalase by using Zr(IV)-modified collagen fiber as the supporting matrix. Process Biochem 46:2187–2193
Wang P, Qi C, Yu Y, Yuan J, Cui L, Tang G, Wang Q, Fan X (2015) Covalent immobilization of catalase onto regenerated silk fibroins via tyrosinase-catalyzed cross-linking. Appl Biochem Biotechnol 177:472–485
Feng Q, Wang Q, Tang B, Wie A, Wang X, Wie Q, Huang F, Cai Y, Hou D, Bi S (2013) Immobilization of catalases on amidoxime polyacrylonitrile nanofibrous membranes. Polym Int 62:251–256
Wang F, Guo C, Yang L-R, Liu C-Z (2013) Magnetic mesoporous silica nanoparticles: fabrication and their laccase immobilization performance. Bioresour Technol 101:8931–8935
Netto CGCM, Toma HE, Andrade LH (2013) Superparamagnetic nanoparticles as versatile carriers and supporting materials for enzymes. J Mol Catal B 85–86:71–92
Woo E-J, Kwon H-S, Lee C-H (2015) Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization. Chem Eng J 274:1–8
Romo-Sanchez S, Arevalo-Villena M, Romero EG, Ramirez HL, Perez AB (2014) Immobilization of β-glucosidase and its application for enhancement of aroma precursors in muscat wine. Food Bioprocess Technol 7:1381–1392
Wang Z-G, Ke B-B, Xu Z-K (2007) Covalent immobilization of redox enzyme on electrospun nonwoven poly(acrylonitrile-co-acrylic acid) nanofiber mesh filled with carbon nanotubes: a comprehensive study. Biotechnol Bioeng 97:708–720
Basak E, Aydemir T, Dincer A, Becerik SC (2013) Comperative study of catalase immobilization on chitosan, magnetic chitosan and chitosan-clay composite beads. Artif Cells Nanomed Biotechnol 41:408–413
Mateo C, Abian O, Fernandez-Lafuente R, Guisan JM (2000) Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment. Enzyme Microb Technol 26:509–515
Bayramoglu G, Akbulut A, Ozalp VC, Arica MY (2015) Immobilized lipase on microporous biosilica for enzymatic transesterification of algal oil. Chem Eng Res Design 95:12–21
Wu H, Liang Y, Shi J, Wang X, Yang D, Jiang Z (2013) Enhanced stability of catalase covalently immobilized on functionalized titania submicrospheres. Mater Sci Eng C 33:1438–1445
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Bayramoglu, G., Arica, M.Y., Genc, A. et al. A facile and efficient method of enzyme immobilization on silica particles via Michael acceptor film coatings: immobilized catalase in a plug flow reactor. Bioprocess Biosyst Eng 39, 871–881 (2016). https://doi.org/10.1007/s00449-016-1566-0
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DOI: https://doi.org/10.1007/s00449-016-1566-0