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Sensitive change of iso-branched fatty acid (iso-15:0) in Bacillus pumilus PAMC 23174 in response to environmental changes

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Abstract

In this study, the environmental adaptive metabolic processes were investigated using a psychrotrophic polar bacterium Bacillus pumilus PAMC 23174 in response to various temperatures and nutrients, especially in regard to the synthesis of fatty acids. Fatty acid methyl ester analysis was performed using gas chromatography–mass spectrometry and we found that a sensitive changes in iso-branched fatty acid (iso-15:0) synthesis occurred when adjusting the nutritional ratio of branched chain fatty acids (anteiso/iso) with different temperatures, resulting in a change in the balance of anteiso- and iso-form fatty acids. We also observed that this Arctic bacterium preferred amino acid leucine for the synthesis of fatty acids. The increased and decreased synthesis of iso-form fatty acids in response to different temperatures and leucine preference, changes the fatty acid ratio in bacteria, which further affects the membrane fluidity and it is also directly correlated with survival of bacteria in an extreme environment. Hence, this study suggests that B. pumilus PAMC 23174 is a potential model organism for the analysis of the unique ecological adaptations of polar bacteria in changing and the extreme environments.

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Acknowledgments

The study was partially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015M1A5A1046168), (NRF-2015M1A5A1037196) and Korea Polar Research Institute (PE15030). This research was supported by Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (1201349190011) and the Energy Efficiency & Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea Government Ministry of Trade, Industry and Energy (20133030000300).

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    Authors and Affiliations

    1. Department of Biological Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea

      Da-Hye Yi, Ganesan Sathiyanarayanan, Hyung Min Seo, Jung-Ho Kim, Shashi Kant Bhatia & Yung-Hun Yang

    2. Chemical Engineering, Soongsil University, 511 Sangdo-dong, Seoul, 156-743, South Korea

      Yun-Gon Kim

    3. Food Ingredients Center, Foods R and D, CheilJedang, Guro-dong, Guro-Gu, Seoul, 152-051, South Korea

      Sung-Hee Park

    4. Division of Life Sciences, Korea Polar Research Institute, 12 Gaetbeol-ro, Yeonsu-gu, Incheon, 406-840, South Korea

      Ji-Young Jung & Yoo Kyung Lee

    5. Microbial Carbohydrate Resource Bank, Konkuk University, Seoul, 143-701, South Korea

      Yung-Hun Yang

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    1. Da-Hye Yi
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    2. Ganesan Sathiyanarayanan
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    Correspondence to Yung-Hun Yang.

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    D.-H. Yi and G. Sathiyanarayanan contributed equally to this work.

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    Yi, DH., Sathiyanarayanan, G., Seo, H.M. et al. Sensitive change of iso-branched fatty acid (iso-15:0) in Bacillus pumilus PAMC 23174 in response to environmental changes. Bioprocess Biosyst Eng 39, 159–167 (2016). https://doi.org/10.1007/s00449-015-1500-x

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    • DOI: https://doi.org/10.1007/s00449-015-1500-x

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