Skip to main content
Log in

Effects of fed-batch and continuous fermentations on human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Lysozyme is a lytic enzyme, which has antimicrobial activity. It has been used for food and pharmaceutical applications. This study was undertaken to evaluate fed-batch and continuous fermentations for the human lysozyme production in biofilm reactor. Results showed that addition of lactose the mid-log phase to make the concentration back to the initial level generates higher lysozyme production (177 U/ml) compared with lactose addition in late-log phase (174 U/ml) (p < 0.05). Moreover, fed-batch fermentation with glucose as initial carbon source and continuous addition of lactose with 0.6 ml/min for 10 h demonstrated significantly higher lysozyme production (187 U/ml) compared to the batch fermentation (173 U/ml) (p < 0.05). In continuous fermentation, biofilm reactor provided significantly higher productivity (7.5 U/ml/h) compared to the maximum productivity in suspended cell bioreactor (4 U/ml/h), because the biofilm reactor provided higher cell density at higher dilution rate compared to suspended cell reactor (p < 0.05).

This is a preview of subscription content, log in via an institution to check access.

Access this article

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Yoshimura K, Toibana A, Kikuchi K, Kobayashi M, Hayakawa T, Nakahama K, Kikuchi M, Ikehara M (1987) Differences between Saccharomyces cerevisiae and Bacillus subtilis in secretion of human lysozyme. Biochem Biophys Res Commun 145(2):712–718

    Article  CAS  Google Scholar 

  2. Naudi AS (2000) Natural food antimicrobial systems. CRC Press, New York

    Google Scholar 

  3. Pichler WJ, Campi P (1992) Allergy to lysozyme/egg white-containing vaginal suppositories. Ann Allergy 69(6):521–525

    CAS  Google Scholar 

  4. Faure A, Jollès P (1970) Immunological reactivity between human and bovine alpha-lactalbumins and several lysozymes of human origin and from bird egg whites. Comptes Rendus Hebdomadaires des Seances de L Academie des Sciences D Sciences Naturelles 271:1916–1918

    CAS  Google Scholar 

  5. Yu Z, Meng Q, Yu H, Fan B, Yu S, Fei J, Wang L, Dai Y, Li N (2006) Expression and bioactivity of recombinant human lysozyme in the milk of transgenic mice. J Dairy Sci 89:2911–2918

    Article  CAS  Google Scholar 

  6. Muraki M, Jigami Y, Tanaka H, Kishimoto F, Agui H, Ogino S (1985) Short communication expression of synthetic human lysozyme gene in Escherichia coli. Agric Biol Chem 49(9):2829–2831

    Article  CAS  Google Scholar 

  7. Choi S, Paik H, Lee S, Nihira T, Hwang Y (2004) Enhanced productivity of human lysozyme by pH-controlled batch fermentation of recombinant Saccharomyces cerevisiae. J Biosci Bioeng 98(2):132–135

    Article  CAS  Google Scholar 

  8. Iwata T, Tanaka R, Seustsugu M, Ishibashi M, Tonkunga H, Kikuchi M, Tokunga M (2004) Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis. Biotechnol Lett 26:1803–1808

    Article  CAS  Google Scholar 

  9. Maullu C, Lampis G, Basile T, Ingiani A, Rossolini CM, Pompei R (1999) Production enriched lysozyme biomass from cheese industry by products. J Appl Microbiol 86:182–186

    Article  CAS  Google Scholar 

  10. Huang EL, Demirci A (2009) Enhanced human lysozyme production by Kluyveromyces lactis. Food Bioprocess Technol 2:222–228

    Article  CAS  Google Scholar 

  11. Ercan D, Demirci A (2013) Production of human lysozyme in biofilm reactor and optimization of growth parameter s of Kluyveromyces lactis K7. Appl Microbiol Biotechnol 97:6211–6221

    Article  CAS  Google Scholar 

  12. Ercan D, Demirci A (2014) Enhanced human lysozyme production in biofilm reactor by Kluyveromyces lactis K7. Biochem Eng J 92:2–8

    Article  CAS  Google Scholar 

  13. Demirci A, Pongtharangkul T, Pometto AL III (2007) Chapter 8: applications of biofilm reactors for production of value-added products by microbial fermentation. In: Blaschek HP, Wang HH, Agle ME (eds) Biofilms in the food environment. Blackwell Publishing and The Institute of Food Technologists, Iowa, pp 167–189

    Google Scholar 

  14. Demirci A, Pometto AL III, Ho K-LC (1997) Ethanol production by Saccharomyces cerevisiae in biofilm reactors. J Ind Microbiol Biotechnol 19:299–304

    Article  CAS  Google Scholar 

  15. Demirci A, Pometto AL III (1995) Repeated-batch fermentation in biofilm reactors with plastic-composite supports for lactic acid production. Appl Microbiol Biotechnol 44:585–589

    Article  Google Scholar 

  16. Siso MIG (1994) β-Galactosidase production by Kluyveromyces lactis on milk whey: batch versus fed-batch cultures. Process Biochem 9:565–568

    Article  Google Scholar 

  17. Paciello L, Romano F, de Alteriis E, Parascandola P, Romano V (2010) Glucoamylase by recombinant Kluyveromyces lactis cells: production and modeling of a fed batch bioreactor. Bioprocess Biosyst Eng 33:525–532

    Article  CAS  Google Scholar 

  18. Pometto AL III, Demirci A, Johnson KE (1997) Immobilization of microorganisms on a support made of synthetic polymer and plant material. US patent 5,595,893

  19. Richard MP, Ramesh CC, Khem MS (1965) A rapid and sensitive assay of muramidase. Proc Soc Exp Biol Med 119:384–386

    Article  Google Scholar 

  20. Rossolini GM, Riccio ML, Gallo E, Galeotti CL (1992) Kluyveromyces lactis rDNA as a target for multiple integration by homologous recombination. Gene 119:75–81

    Article  CAS  Google Scholar 

  21. Ibrahim HR, Higashiguchi S, Koketsu M, Juneja LR, Kim M, Yamamoto T, Sugimoto Y, Aoki T (1996) Partially unfolded lysozyme at neutral pH agglutinates and kills Gram-negative and Gram-positive bacteria through membrane damage mechanism. J Agric Food Chem 44:3799–3806

    Article  CAS  Google Scholar 

  22. Ibrahim HR, Matsuzaki T, Aoki T (2001) Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function. Fed Eur Biochem Soc Lett 506:27–32

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by Turkish Ministry of Education by providing scholarship to Duygu Ercan and the Pennsylvania Experiment Station. We also thank Dr. Anthony L. Pometto III for helping manufacturing plastic composite supports. Moreover, we thank Hilmar Ingredients Inc. (Hilmar, CA) for providing lactose.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ali Demirci.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ercan, D., Demirci, A. Effects of fed-batch and continuous fermentations on human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors. Bioprocess Biosyst Eng 38, 2461–2468 (2015). https://doi.org/10.1007/s00449-015-1483-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-015-1483-7

Keywords

Navigation