Abstract
Lysozyme is a lytic enzyme, which has antimicrobial activity. It has been used for food and pharmaceutical applications. This study was undertaken to evaluate fed-batch and continuous fermentations for the human lysozyme production in biofilm reactor. Results showed that addition of lactose the mid-log phase to make the concentration back to the initial level generates higher lysozyme production (177 U/ml) compared with lactose addition in late-log phase (174 U/ml) (p < 0.05). Moreover, fed-batch fermentation with glucose as initial carbon source and continuous addition of lactose with 0.6 ml/min for 10 h demonstrated significantly higher lysozyme production (187 U/ml) compared to the batch fermentation (173 U/ml) (p < 0.05). In continuous fermentation, biofilm reactor provided significantly higher productivity (7.5 U/ml/h) compared to the maximum productivity in suspended cell bioreactor (4 U/ml/h), because the biofilm reactor provided higher cell density at higher dilution rate compared to suspended cell reactor (p < 0.05).
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Acknowledgments
This work was supported in part by Turkish Ministry of Education by providing scholarship to Duygu Ercan and the Pennsylvania Experiment Station. We also thank Dr. Anthony L. Pometto III for helping manufacturing plastic composite supports. Moreover, we thank Hilmar Ingredients Inc. (Hilmar, CA) for providing lactose.
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Ercan, D., Demirci, A. Effects of fed-batch and continuous fermentations on human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors. Bioprocess Biosyst Eng 38, 2461–2468 (2015). https://doi.org/10.1007/s00449-015-1483-7
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DOI: https://doi.org/10.1007/s00449-015-1483-7