Anaerobic removal of 1-methoxy-2-propanol under ambient temperature in an EGSB reactor


Two laboratory-scale expanded granular sludge bed (EGSB) reactors were operated at 18 and 25 °C, respectively, for the treatment of synthetic wastewater composed of ethanol and 1-methoxy-2-propanol (M2P) in a mass ratio of 4:1. Reactors were operated first with continuous wastewater supply and after with discontinuous substrate supply (5 days a week, 16 h a day) to simulate shift working conditions. Under continuous wastewater supply chemical oxygen demand (COD), removal efficiency higher than 95 % was achieved at the end of the trial applying organic loading rates (OLR) of 29 and 43 kg COD m−3 day−1 at 18 and 25 °C; thus, corresponding to M2P OLR of 6.4 and 9.3 kg COD m−3 day−1, respectively. During intermittent supply of substrate, good performance was recorded at both temperatures with an OLR of 30 kg COD m−3 day−1 (M2P OLR of 6.6 kg COD m−3 day−1). After 56 h without substrate supply, a decline in methane yield of 15–30 % was observed due to the deactivation of the biomass. Specific methanogenic activity (SMA) assays were carried out at the end of the experiments. SMA values using 1-methoxy-2-propanol as substrate were 24.3 and 7.8 ml CH4 gVSS−1 day−1 at 25 °C and at 18 °C, respectively. This is the first attempt to investigate the removal of 1-methoxy-2-propanol by EGSB reactors.

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  1. 1.

    Aydin AF, Ersahin ME, Dereli RK, Sarikaya HZ, Ozturk I (2010) Long-term anaerobic treatability studies on opium alkaloids industry effluents. J Environ Sci Health Part A-Toxic/Hazard Subst Environ Eng 45:192–200

    CAS  Article  Google Scholar 

  2. 2.

    European Commision (2006) European Union Risk Assessment Report

  3. 3.

    Enright A, McHugh S, Collins G, O’Flaherty V (2005) Low-temperature anaerobic biological treatment of solvent-containing pharmaceutical wastewater. Water Res 39:4587–4596

    CAS  Article  Google Scholar 

  4. 4.

    Scully C, Collins G, O’Flaherty V (2006) Anaerobic biological treatment of phenol at 9.5–15 °C in an expanded granular sludge bed (EGSB)-based bioreactor. Water Res 40:3737–3744

    CAS  Article  Google Scholar 

  5. 5.

    Enright A, Collins G, O’Flaherty V (2007) Low-temperature anaerobic biological treatment of toluene-containing wastewater. Water Res 41:1465–1472

    CAS  Article  Google Scholar 

  6. 6.

    van Lier JB (2008) High-rate anaerobic wastewater treatment: diversifying from end-of-the-pipe treatment to resource-oriented conversion techniques. Water Sci Technol 57:1137–1148

    Article  Google Scholar 

  7. 7.

    del Pozo R, Diez V, Salazar G (2002) Start-up of a pilot-scale anaerobic fixed film reactor at low temperature treating slaughterhouse wastewater. Water Sci Technol 46:215–221

    Google Scholar 

  8. 8.

    Siggins A, Enright A, O’Flaherty V (2011) Temperature dependent (37–15 °C) anaerobic digestion of a trichloroethylene-contaminated wastewater. Bioresour Technol 102:7645–7656

    CAS  Article  Google Scholar 

  9. 9.

    Rajeshwari KV, Balakrishnan M, Kansal A, Lata K, Kishore VVN (2000) State-of-the-art of anaerobic digestion technology for industrial wastewater treatment. Renew Sustain Energy Rev 4:135–156

    CAS  Article  Google Scholar 

  10. 10.

    Lettinga G, Rebac S, Zeeman G (2001) Challenge of psychrophilic anaerobic wastewater treatment. Trends Biotechnol 19:363–370

    CAS  Article  Google Scholar 

  11. 11.

    Kashyap DR, Dadhich KS, Sharma SK (2003) Biomethanation under psychrophilic conditions: a review. Bioresour Technol 87:147–153

    CAS  Article  Google Scholar 

  12. 12.

    Kettunen RH, Rintala JA (1997) The effect of low temperature (5–29 °C) and adaptation on the methanogenic activity of biomass. Appl Microbiol Biotechnol 48:570–576

    CAS  Article  Google Scholar 

  13. 13.

    Zoutberg GR, deBeen P (1997) The Biobed(R) EGSB (expanded granular sludge bed) system covers shortcomings of the upflow anaerobic sludge blanket reactor in the chemical industry. Water Sci Technol 35:183–188

    CAS  Article  Google Scholar 

  14. 14.

    Seghezzo L, Zeeman G, van Lier JB, Hamelers HVM, Lettinga G (1998) A review: the anaerobic treatment of sewage in UASB and EGSB reactors. Bioresour Technol 65:175–190

    CAS  Article  Google Scholar 

  15. 15.

    Leitao RC, van Haandel AC, Zeeman G, Lettinga G (2006) The effects of operational and environmental variations on anaerobic wastewater treatment systems: a review. Bioresour Technol 97:1105–1118

    CAS  Article  Google Scholar 

  16. 16.

    Coelho NM, Rodrigues AA, Arroja LM, Capela IF (2007) Effect of non-feeding period length on the intermittent operation of UASB reactors treating dairy effluents. Biotechnol Bioeng 96:244–249

    CAS  Article  Google Scholar 

  17. 17.

    Eaton AD, Clesceri LS, Greenberg AE, Franson MAH, American Public Health Association (1998) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, DC

  18. 18.

    Kato M, Field J, Versteeg P, Lettinga G (1994) Feasibility of expanded granular sludge bed reactors for the anaerobic treatment of low-strength soluble wastewaters. Biotechnol Bioeng 44:469–479

    CAS  Article  Google Scholar 

  19. 19.

    Speranza G, Mueller B, Orlandi M, Morelli C, Manitto P, Schink B (2002) Mechanism of anaerobic ether cleavage—Conversion of 2-phenoxyethanol to phenol and acetaldehyde by Acetobacterium sp. J Biol Chem 277:11684–11690

    CAS  Article  Google Scholar 

  20. 20.

    Kawai F (2002) Microbial degradation of polyethers. Appl Microbiol Biotechnol 58:30–38

    CAS  Article  Google Scholar 

  21. 21.

    Nadais H, Capela I, Arroja L, Duarte A (2005) Optimum cycle time for intermittent UASB reactors treating dairy wastewater. Water Res 39:1511–1518

    CAS  Article  Google Scholar 

  22. 22.

    van den Berg L (1977) Effect of temperature on growth and activity of a methanogenic culture utilizing acetate. Can J Microbiol 23:898–902

    Article  Google Scholar 

  23. 23.

    Upadhyay U, Kumar P, Mehrotra I (2008) Anaerobic degradation of benzoate: batch studies. Bioresour Technol 99:6861–6865

    CAS  Article  Google Scholar 

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This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No. 284949. Financial support from Ministerio de Economía y Competitividad (Project CTM2014-54517) and Generalitat Valenciana (PROMETEO/2013/053), Spain, is also acknowledged. C. Lafita has a FPI grant from Ministerio de Economía y Competitividad, Spain.

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Lafita, C., Penya-roja, J.M. & Gabaldón, C. Anaerobic removal of 1-methoxy-2-propanol under ambient temperature in an EGSB reactor. Bioprocess Biosyst Eng 38, 2137–2146 (2015).

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  • 1-Methoxy-2-propanol
  • EGSB
  • Packaging wastewater
  • Ambient temperature