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Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products

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Abstract

The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8–10 IU gds−1 of FPA and 15.6–17.8 IU gds−1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds−1 FPA and 13.5 IU gds−1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe+2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast®. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.

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Acknowledgments

We thank Dr. Irina Druzhinina (Technological University of Vienna, Austria) for generously providing the Trichoderma reesei and Trichoderma parareesei strains employed in these investigations. This work was supported by an IDB OC/AR loan with a PICT start-up 2010-1312 grant from the FONCyT–MinCyT of Argentina (issued to E. Albertó) and the PGSYS program (issued to M. Fernández-Lahore). Dr. Donald F. Haggerty, a retired career investigator and native English speaker, edited the final version of the manuscript.

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    Authors and Affiliations

    1. Instituto de Investigaciones Biotecnológicas–Instituto Tecnológico Chascomús (IIB–INTECH, UNSAM–CONICET), Universidad de San Martín, Av. 25 de Mayo y Francia, Campus UNSAM, 1650, San Martín, Buenos Aires, Argentina

      Gastón E. Ortiz & Edgardo O. Albertó

    2. Downstream Processing Laboratory, Jacobs University Bremen, 28759, Bremen, Germany

      Gastón E. Ortiz, María E. Guitart & Marcelo Fernández-Lahore

    3. Institute of Chemistry and Biological Physicochemistry (IQUIFIB), School of Pharmacy and Biochemistry, University of Buenos Aires and National Research Council (CONICET), Junín 956, Buenos Aires, Argentina

      María E. Guitart

    4. Research and Development Center for Industrial Fermentations (CINDEFI) (UNLP, CONICET La Plata), Calle 47 y 115, 1900, La Plata, Argentina

      Sebastián F. Cavalitto

    5. Centro de Investigación y Desarrollo en Biotecnología Industrial, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, Edificio 51, San Martín, Buenos Aires, Argentina

      Martín Blasco

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    1. Gastón E. Ortiz
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    3. Sebastián F. Cavalitto
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    4. Edgardo O. Albertó
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    Correspondence to Gastón E. Ortiz.

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    E. O. Albertó, M. Fernández-Lahore, M. Blasco equally contributed to this work as co-senior authors.

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    Ortiz, G.E., Guitart, M.E., Cavalitto, S.F. et al. Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products. Bioprocess Biosyst Eng 38, 2117–2128 (2015). https://doi.org/10.1007/s00449-015-1451-2

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    • DOI: https://doi.org/10.1007/s00449-015-1451-2

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