Enhanced ethanol production by fermentation of Gelidium amansii hydrolysate using a detoxification process and yeasts acclimated to high-salt concentration

Abstract

A total monosaccharide concentration of 59.0 g/L, representing 80.1 % conversion of 73.6 g/L total fermentable sugars from 160 g dw/L G. amansii slurry was obtained by thermal acid hydrolysis and enzymatic hydrolysis. Subsequent adsorption treatment using 5 % activated carbon with an adsorption time of 2 min was used to prevent the inhibitory effect of 5-hydroxymethylfurfural (HMF) >5 g/L in the medium. Ethanol production decreased with increasing salt concentration using C. tropicalis KCTC 7212 non-acclimated or acclimated to a high concentration of salt. Salt concentration of 90 psu was the maximum concentration for cell growth and ethanol production. The levels of ethanol production by C. tropicalis non-acclimated or acclimated to 90 psu high-salt concentration were 13.8 g/L with a yield (YEtOH) of 0.23, and 26.7 g/L with YEtOH of 0.45, respectively.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2013R1A1A2059095).

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Correspondence to Sung-Koo Kim.

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Ra, C.H., Jung, J.H., Sunwoo, I.Y. et al. Enhanced ethanol production by fermentation of Gelidium amansii hydrolysate using a detoxification process and yeasts acclimated to high-salt concentration. Bioprocess Biosyst Eng 38, 1201–1207 (2015). https://doi.org/10.1007/s00449-015-1362-2

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Keywords

  • Acclimated yeast
  • Ethanol production
  • Salt
  • Gelidium amansii
  • Candida tropicalis KCTC 7212