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Cyclodextrin glycosyltransferase production by free cells of Bacillus circulans DF 9R in batch fermentation and by immobilized cells in a semi-continuous process

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Abstract

Cyclodextrin glycosyltransferase (CGTase) catalyzes starch conversion into cyclic or linear oligosaccharides, important industrial products for the complexation of non-polar substances. In this work, conditions to increase CGTase production from Bacillus circulans strain DF 9R were optimized by two systems. On one hand, free cells were grown in batch fermentation experiments to optimize aeration and pH. The highest activity (1.47 ± 0.21 U ml−1) was achieved after 48 h of growth, aeration of 1.5 vvm and pH regulated to 7.6. On the other hand, bacterial cells were immobilized on loofa and synthetic sponge, and used for CGTase production in a semi-continuous process. An initial biomass of 30 mg of lyophilized cells and an immobilization time of 24 h with loofa or synthetic sponge were enough to achieve increased production of CGTase: 0.91 ± 0.10 and 0.95 ± 0.11 U ml−1, respectively. Sponges with immobilized bacteria were reused in 12 successive cycles. Besides, in our conditions, CGTase was not adsorbed onto the supports used for immobilization, which ensured the total recovery of the enzyme from the culture medium. The two CGTase production processes studied showed similar productivity and could be potentially scaled up.

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Acknowledgments

We thank the financial support of the following institutions: Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), PICT 2013-0880 préstamo BID, Universidad Nacional de Luján and Ministerio de Ciencia, Tecnología e Innovación Productiva (MINCyT) from Argentina, and Universidade Estadual de Maringá and Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES) from Brazil. HC is a career member of the Argentine Council of Scientific and Technical Research (CONICET). We thank professor Victoria Eusevi for language supervision.

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Authors and Affiliations

  1. Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, 6700, Buenos Aires, Republic Argentina

    Hernán Costa, Jorgelina Rodríguez Gastón, Julia Lara & Susana Alicia Ferrarotti

  2. Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil

    Camila Ortiz Martinez, Cristiane Moriwaki & Graciette Matioli

Authors
  1. Hernán Costa
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  2. Jorgelina Rodríguez Gastón
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  3. Julia Lara
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  4. Camila Ortiz Martinez
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  5. Cristiane Moriwaki
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  6. Graciette Matioli
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  7. Susana Alicia Ferrarotti
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Correspondence to Susana Alicia Ferrarotti.

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Costa, H., Gastón, J.R., Lara, J. et al. Cyclodextrin glycosyltransferase production by free cells of Bacillus circulans DF 9R in batch fermentation and by immobilized cells in a semi-continuous process. Bioprocess Biosyst Eng 38, 1055–1063 (2015). https://doi.org/10.1007/s00449-014-1347-6

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  • DOI: https://doi.org/10.1007/s00449-014-1347-6

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