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New strategy to apply perfluorodecalin as an oxygen carrier in lipase production: minimisation and reuse

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Abstract

A novel strategy for the production of lipase by Bacillus sp. ITP-001 in a stirred tank fermenter using perfluorodecalin (PFD) was studied. Firstly, a response surface methodology 22 with three central points was employed to optimise the effect of agitation speed and aeration rate in lipase production. According to the response from the experimental designs, 300 rpm (revolutions per minute) and 0.5 vvm (air volume/liquid volume per minute) were found to provide the best condition (lipolytic activity: LA = 3,140.76 U mL−1). Then, the influence of PFD concentration on the fermentation process was evaluated. Incorporation of PFD at all concentrations above 1 % had no statistically significant influence on lipase production, that is, the previous optimisation allowed the reduction of the amount of PFD added besides increasing lipase production. Furthermore, PFD could be used in three sequential fermentations without altering the statistical production of lipase, reducing by 67 % the cost of PFD addition.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Engenharia de Processos, Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia, Aracaju, 49032-490, Brazil

    Erika Souza Vieira, Tâmara Karoline de Oliveira Fontes, Hofsky Vieira Alexandre, Cleide Mara Faria Soares & Álvaro Silva Lima

  2. Instituto de Tecnologia e Pesquisa, Av. Murilo Dantas 300, Farolândia, Aracaju, 49032-490, Brazil

    Matheus Mendonça Pereira, Hofsky Vieira Alexandre, Cleide Mara Faria Soares & Álvaro Silva Lima

  3. Núcleo de Engenharia de Produção, Universidade Federal de Sergipe, Av. Marechal Rondon s/n, Jardim Rosa Elze, Sao Cristovao, 49100-000, Brazil

    Daniel Pereira da Silva

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  1. Erika Souza Vieira
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  2. Tâmara Karoline de Oliveira Fontes
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  3. Matheus Mendonça Pereira
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  4. Hofsky Vieira Alexandre
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  6. Cleide Mara Faria Soares
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  7. Álvaro Silva Lima
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Correspondence to Álvaro Silva Lima.

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Vieira, E.S., de Oliveira Fontes, T.K., Pereira, M.M. et al. New strategy to apply perfluorodecalin as an oxygen carrier in lipase production: minimisation and reuse. Bioprocess Biosyst Eng 38, 721–728 (2015). https://doi.org/10.1007/s00449-014-1312-4

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  • DOI: https://doi.org/10.1007/s00449-014-1312-4

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