Abstract
An aerobic denitrifier was isolated from activated sludge and the isolate possessed an average nitrate removal efficiency of 95.8 % in 24 h, with the maximum nitrite accumulation of 25.6 mg/l. The bacterium could not conduct heterotrophic nitrification. The results of PCR indicated that the strain lacked the gene of amoA. The phylogenetic tree indicated a clear evolutionary divergence that Pseudomonas stutzeri C3 belonged to the group of strains that could not exhibit ammonia oxidation.
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Acknowledgments
This research was financially supported by the open fund of State Key Lab of Urban Water Resources and Environment (HIT) (No. QAK201014), the National Natural Science Foundation of China (NSFC) (No. 51378400), and Major Science and Technology Program for Water Pollution Control and Treatment (No. 2009ZX07317-008-003).
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Ji, B., Yang, K., Wang, H. et al. Aerobic denitrification by Pseudomonas stutzeri C3 incapable of heterotrophic nitrification. Bioprocess Biosyst Eng 38, 407–409 (2015). https://doi.org/10.1007/s00449-014-1271-9
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DOI: https://doi.org/10.1007/s00449-014-1271-9