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The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m3 fermenter

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Abstract

In this paper, glucose respiratory quotient (RQ)-feedback control was developed for erythromycin production with a recombinant strain Saccharopolyspora erythraea ZL1004. RQ was confirmed to be an ideal online parameter for regulating glucose feed rate. Through feeding glucose to control RQ at 0.85 during 45–100 h and 0.95 during 100–185 h, erythromycin titer and erythromycin A concentration were reached 11.88 and 8.82 g l−1 in 50 l fermenter, which were increased by 8.3 and 6.1 % as compared to that with glucose pH-feedback control, respectively. When glucose RQ-feedback control was scaled up to 372-m3 fermenter, erythromycin titer and erythromycin A concentration at 155 h were reached 9.12 and 7.12 g l−1, respectively, which were 10.5 and 9.4 % higher than that with the original technology (glucose pH-feedback control). To the best of our knowledge, this is the first report on the successful application of glucose RQ-feedback control in erythromycin production, especially in 372-m3 fermenter.

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Acknowledgments

This work was financially supported by a grant from the Major State Basic Research Development Program of China (973 Program, No. 2012CB721006), National Natural Science Foundation of China (No. 21276081), the National Scientific and Technological Major Special Project (Significant Creation of New drugs, No. 2011ZX09203-001-03), and Research Fund for the Doctoral Program of Higher Education of China (No. 20110074110015).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. Box 329, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Yong Chen, Zejiang Wang, Ju Chu & Yingping Zhuang

  2. Food Science and Engineering College, Qingdao Agriculture University, Qingdao, 266109, Shandong, People’s Republic of China

    Yong Chen

  3. Shanghai Xuhui District Central Hospital, No. 966 Huaihai Zhong Road, Shanghai, 200031, People’s Republic of China

    Beili Xi

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  1. Yong Chen
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  2. Zejiang Wang
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  3. Ju Chu
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  4. Beili Xi
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  5. Yingping Zhuang
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Correspondence to Ju Chu.

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Chen, Y., Wang, Z., Chu, J. et al. The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m3 fermenter. Bioprocess Biosyst Eng 38, 105–112 (2015). https://doi.org/10.1007/s00449-014-1248-8

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  • DOI: https://doi.org/10.1007/s00449-014-1248-8

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