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Biosynthesis of fluorescent gold nanoparticles using an edible freshwater red alga, Lemanea fluviatilis (L.) C.Ag. and antioxidant activity of biomatrix loaded nanoparticles

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Abstract

Biosynthesis of gold nanoparticles has been accomplished via reduction of an aqueous chloroauric acid solution with the dried biomass of an edible freshwater epilithic red alga, Lemanea fluviatilis (L.) C.Ag., as both reductant and stabilizer. The synthesized nanoparticles were characterized by UV–visible, powder X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), and dynamic light scattering (DLS) studies. The UV–visible spectrum of the synthesized gold nanoparticles showed the surface plasmon resonance (SPR) at around 530 nm. The powder XRD pattern furnished evidence for the formation of face-centered cubic structure of gold having average crystallite size 5.9 nm. The TEM images showed the nanoparticles to be polydispersed, nearly spherical in shape and have sizes in the range 5–15 nm. The photoluminescence spectrum of the gold nanoparticles excited at 300 nm showed blue emission at around 440 nm. Gold nanoparticles loaded within the biomatrix studied using a modified 2,2-diphenyl-1-picrylhydrazyl (DPPH) method exhibited pronounced antioxidant activity.

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Acknowledgments

Authors thank SAIF, NEHU, Shillong, for TEM facilities. BS thanks University Grants Commission and MT thanks Department of Biotechnology, Government of India for Research Fellowships. We thank DBT e-Library Consortium (DeLCON) of Bioinformatics Centre, Assam University, Silchar.

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Authors and Affiliations

  1. Department of Ecology and Environmental Sciences, Assam University, Silchar, 788011, Assam, India

    Banasree Sharma, Moirangthem Thajamanbi & Jayashree Rout

  2. Department of Chemistry, Assam University, Silchar, 788011, Assam, India

    Debraj Dhar Purkayastha & Chira R. Bhattacharjee

  3. Nano-Materials Laboratory, Department of Chemistry, BITS-Pilani, K.K. Birla Goa Campus, Zuarinagar, 403726, Goa, India

    Subhenjit Hazra & Narendra Nath Ghosh

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  1. Banasree Sharma
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  2. Debraj Dhar Purkayastha
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Correspondence to Jayashree Rout.

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Sharma, B., Purkayastha, D.D., Hazra, S. et al. Biosynthesis of fluorescent gold nanoparticles using an edible freshwater red alga, Lemanea fluviatilis (L.) C.Ag. and antioxidant activity of biomatrix loaded nanoparticles. Bioprocess Biosyst Eng 37, 2559–2565 (2014). https://doi.org/10.1007/s00449-014-1233-2

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  • DOI: https://doi.org/10.1007/s00449-014-1233-2

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