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Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol–gel support

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Abstract

This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.

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Acknowledgments

The authors gratefully thank to Ministry of Education, Malaysia and Universiti Malaysia Pahang for financial support through GRS 120347.

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Authors and Affiliations

  1. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, Gambang, 26300, Kuantan, Pahang Darul Makmur, Malaysia

    Ponnarasy Ganasen, Maksudur Rahman Khan & Mohd Sabri Mahmud

  2. Department of BGPS, Sul Ross State University, Alpine, TX, 97832, USA

    M. Abul Kalam

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  1. Ponnarasy Ganasen
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  2. Maksudur Rahman Khan
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  3. M. Abul Kalam
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  4. Mohd Sabri Mahmud
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Correspondence to Maksudur Rahman Khan.

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Ganasen, P., Khan, M.R., Kalam, M.A. et al. Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol–gel support. Bioprocess Biosyst Eng 37, 2353–2359 (2014). https://doi.org/10.1007/s00449-014-1213-6

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  • DOI: https://doi.org/10.1007/s00449-014-1213-6

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