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Enhancement of tannase production by Lactobacillus plantarum CIR1: validation in gas-lift bioreactor

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Abstract

The optimization of tannase production by Lactobacillus plantarum CIR1 was carried out following the Taguchi methodology. The orthogonal array employed was L18 (21 × 35) considering six important factors (pH and temperature, also phosphate, nitrogen, magnesium, and carbon sources) for tannase biosynthesis. The experimental results obtained from 18 trials were processed using the software Statistical version 7.1 using the character higher the better. Optimal culture conditions were pH, 6; temperature, 40 °C; tannic acid, 15.0 g/L; KH2PO4, 1.5 g/L; NH4Cl, 7.0 g/L; and MgSO4, 1.5 g/L which were obtained and further validated resulting in an enhance tannase yield of 2.52-fold compared with unoptimized conditions. Tannase production was further carried out in a 1-L gas-lift bioreactor where two nitrogen flows (0.5 and 1.0 vvm) were used to provide anaerobic conditions. Taguchi methodology allowed obtaining the optimal culture conditions for the production of tannase by L. plantarum CIR1. At the gas-lift bioreactor the tannase productivity yields increase 5.17 and 8.08-fold for the flow rates of 0.5 and 1.0 vvm, respectively. Lactobacillus plantarum CIR1 has the capability to produce tannase at laboratory-scale. This is the first report for bacterial tannase production using a gas-lift bioreactor.

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Acknowledgments

Author Pedro Aguilar-Zárate appreciates the scholarship provided by the Mexican Council for Science and Technology (CONACYT).

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Authors and Affiliations

  1. Departments of Food Research and Chemical Engineering, School of Chemistry, Universidad Autonoma de Coahuila, Venustiano Carranza S/N Col. República Oriente, 25280, Saltillo, Coahuila, Mexico

    Pedro Aguilar-Zarate, Julio C. Montañez, Ruth E. Belmares-Cerda & Cristobal N. Aguilar

  2. Department of Food Science and Food Technology, Universidad Autónoma Agraria Antonio Narro, Calzada Antonio Narro #1923, Col. Buenavista, 25315, Saltillo, Coahuila, Mexico

    Mario A. Cruz-Hernandez

Authors
  1. Pedro Aguilar-Zarate
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  2. Mario A. Cruz-Hernandez
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  3. Julio C. Montañez
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  4. Ruth E. Belmares-Cerda
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  5. Cristobal N. Aguilar
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Correspondence to Mario A. Cruz-Hernandez or Cristobal N. Aguilar.

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Aguilar-Zarate, P., Cruz-Hernandez, M.A., Montañez, J.C. et al. Enhancement of tannase production by Lactobacillus plantarum CIR1: validation in gas-lift bioreactor. Bioprocess Biosyst Eng 37, 2305–2316 (2014). https://doi.org/10.1007/s00449-014-1208-3

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  • DOI: https://doi.org/10.1007/s00449-014-1208-3

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