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Enhanced production of triterpenoid in submerged cultures of Antrodia cinnamomea with the addition of citrus peel extract

Bioprocess and Biosystems Engineering volume 37, pages 2251–2261 (2014)Cite this article

Abstract

In recent years, Antrodia cinnamomea has become a well-known medicinal mushroom in Taiwan. Triterpenoids are considered one of the most biologically active components found in A. cinnamomea. The aim of this research is to investigate the feasibility of enhancing triterpenoid production in shake flask cultures of A. cinnamomea by adding citrus peel extract. As a result of its containing essential oils, citrus peel extract is inhibitory to mycelial growth. In the experiments, the appropriate adding time is determined to be on day 7. Of the various citrus peel extracts tested, tangerine proves to be the most effective in enhancing polyphenol and triterpenoid production. With an addition of 2 % (v/v), the content and production of total polyphenols rises from 5.95 mg/g DW of the control and 56.73 mg/L to 23.52 mg/g DW and 224.39 mg/L, respectively, on day 28. The production of triterpenoids also increases from 99.93 to 1,028.02 mg/L, for more than a tenfold increase. An optimal level of tangerine peel additive is determined to be around 4 %. Furthermore, when compared with the mycelia of the control culture, the profiles of the HPLC analysis show that the mycelia cultured with the tangerine-peel addition contain more kinds of triterpenoids. This study demonstrates that the addition of citrus peel extract effectively enhances the production of bioactive metabolites in the submerged cultures of A. cinnamomea.

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Acknowledgments

The authors wish to thank the National Science Council of the R. O. C. for financial supports (NSC 102-2221-E-029-018).

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Affiliations

  1. Department of Chemical and Materials Engineering, Tunghai University, Taichung, 40704, Taiwan

    Te-Wei Ma, Yueting Lai & Fan-Chiang Yang

  2. Department of Chemical Engineering, Army Academy, Taoyuan, 32092, Taiwan

    Te-Wei Ma

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  1. Te-Wei Ma
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Correspondence to Fan-Chiang Yang.

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Ma, TW., Lai, Y. & Yang, FC. Enhanced production of triterpenoid in submerged cultures of Antrodia cinnamomea with the addition of citrus peel extract. Bioprocess Biosyst Eng 37, 2251–2261 (2014). https://doi.org/10.1007/s00449-014-1203-8

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  • DOI: https://doi.org/10.1007/s00449-014-1203-8

Keywords

  • Antrodia cinnamomea
  • Triterpenoids
  • Submerged culture
  • Citrus peel extract