Abstract
The purpose of this study was to enhance the economic efficiency of producing bioethanol. Pretreatment solution recycling is expected to increase economic efficiency by reducing the cost of pretreatment and the amount of wastewater. In addition, the production of high-concentration bioethanol could increase economic efficiency by reducing the energy cost of distillation. The pretreatment conditions were 95 °C, 0.72 M NaOH, 80 rpm twin-screw speed, and flow rate of 90 mL/min at 18 g/min of raw biomass feeding for pretreatment solution recycling. The pretreatment with NaOH solution recycling was conducted five times. All of the components and the pretreatment efficiency were similar, despite reuse. In addition, we developed a continuous biomass feeding system for production of high-concentration bioethanol. Using this reactor, the bioethanol productivity was investigated using various pretreated biomass feeding rates in a simultaneous saccharification and fermentation (SSF) process. The maximum ethanol concentration, yield, and productivity were 74.5 g/L, 89.5 %, and 1.4 g/L h, respectively, at a pretreated biomass loading of approximately 25 % (w/v) with an enzyme dosage of 30 FPU g/cellulose. The results presented here constitute an important contribution toward the production of bioethanol from Miscanthus.
Similar content being viewed by others
References
Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA (2006) The path forward for biofuels and biomaterials. Science 311:484–489
Ragauskas AJ, Nagy M, Kim DH, Eckert CA, Hallett JP, Liotta CL (2006) From wood to fuels: integrating biofuels and pulp production. Ind Biotechnol 2:55–65
Sorensen A, Teller PJ, Hilstrom T, Ahring BK (2008) Hydrolysis of Miscanthus for bioethanol production using dilute acid presoaking combined with wet explosion pre-treatment and enzymatic treatment. Bioresour Technol 99:6602–6607
de Vrije T, de Haas GG, Tan GB, Keijsers ERP, Claassen PAM (2002) Pretreatment of Miscanthus for hydrogen production by Thermotoga elfii. Int J Hydrogen Energ 27:1381–1390
Leegood RC, Lea PJ, Adcock MD, Hausler RE (1995) The regulation and control of photorespiration. J Exp Bot 46:1397–1414
Eitzinger J, Kossler C (2002) Microclimatological characteristics of a Miscanthus (Miscanthus cv. giganteus) stand during stable conditions at night in the nonvegetative winter period. Theor Appl Climatol 72:245–257
Himken M, Lammet J, Neukirchen D, Czypionka-Kause U, Olfs H-W (1997) Cultivation of Miscanthus under West European conditions: seasonal changes in dry matter production, nutrient uptake and remobilization. Plant Soil 189:117–126
Lewandowski I, Clifton-Brown JC, Andersson B, Brasch G, Chrsitian DG, Jørgensen U, Jones MB, Riche AB, Schwarz KU, Tayebi K, Teixeira F (2003) Environment and harvest time affects the combustion qualities of Miscanthus genotypes. Agron J 95:1274–1280
Badger PC (2002) Ethanol from cellulose: a general review. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. ASHS Publishers, Alexandria
Converse AO, Ooshima H, Burns DS (1990) Kinetics of enzymatic hydrolysis of lignocellulosic materials based on surface area of cellulose accessible to enzyme and enzyme adsorption on lignin and cellulose. Appl Biochem Biotechnol 24–25:67–73
Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 96:673–686
Patel SJ, Onkarappa R, Ks S (2007) Fungal pretreatment studies on rice husk and bagasse for ethanol production. Electr J Environ Agr Food Chem 6:1921–1926
Kumar P, Barrett DM, Delwiche MJ, Stroeve P (2009) Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Ind Eng Chem Res 48:3713–3729
Díaz MJ, Cara C, Ruiz E, Romero I, Moya M, Castro E (2010) Hydrothermal pre-treatment of rapeseed straw. Bioresour Technol 101(7):2428–2435
Hsu TA (2010) Pretreatment of biomass. In: Wyman CE (ed) Handbook on bioethanol production and utilization (applied energy technology series). Taylor & Francis, Washington, pp 179–212
Dale BE, Henk LL, Shiang M (1985) Fermentation of lignocellulosic materials treated by ammonia fiber explosion. Dev Ind Microb 26:223–233
Kim TH, Lee YY (2005) Pretreatment of corn stover by soaking in aqueous ammonia. Appl Biochem Biotechnol 124:1119–1131
Brownell HH, Yu EKC, Saddler JN (1986) Steam explosion pretreatment of wood: effect of chip size, acid, moisture content, and pressure drop. Biotechnol Bioeng 28:792–801
Tan L, Tang YQ, Nishimura H, Takei S, Morimura S, Kida K (2013) Efficient production of bioethanol from corn stover by pretreatment with a combination of sulfuric acid and sodium hydroxide. Prep Biochem Biotechnol 43(7):682–695
Han M, Kim Y, Kim SW, Choi GW (2011) High efficiency bioethanol production from OPEFB using pilot pretreatment reactor. J Chem Technol Biotechnol 86(12):1527–1534
Lee SH, Teramoto Y, Tanaka N, Endo T (2009) Enzymatic saccharification of woody biomass micro/nanofibrillated by continuous extrusion process I—effect of additives with cellulose affinity. Biores Technol 100(1):275–279
Lee SH, Inoue S, Teramoto Y, Tanaka N, Endo T (2010) Enzymatic saccharification of woody biomass micro/nanofibrillated by continuous extrusion process II—effect of hot-compressed water treatment. Biores Technol 101(24):9645–9649
Han M, Kang KE, Kim Y, Choi GW (2013) High efficiency bioethanol production from barley straw using a continuous pretreatment reactor. Process Biochem 48(3):488–495
Kang KE, Han M, Moon SE, Kang HW, Cha YL, Choi GW (2013) Optimization of alkali-extrusion pretreatment with twin-screw for bioethanol production from Miscanthus. Fuel 109:520–526
Lamsal B, Brijwani K, Alavi S (2010) Extrusion as a thermo-mechanical pre-treatment for lignocellulosic ethanol. Biomass Bioenerg 34(12):1703–1710
Madson PW (2009) Ethanol distillation: the fundamentals. In: Ingledew WM, Kelsall DR, Austin GD, Kluspies C (eds) The alcohol textbook. Nottingham University Publishers, Nottingham, pp 292–297
National Renewable Energy Laboratory, Standard Biomass Analytical Procedures. http://www.nrel.gov/biomass/analytical_procedures.html
Samson R, Mani S, Boddey R, Sokhansanj S, Quesada D, Urquiaga S, Reis V, Lem CH (2005) The potential of C4 perennial grasses for developing a global bioheat industry. Crit Rev Plant Sci 24:461–495
Brosse N, Sannigrahi P, Ragauska A (2009) Pretreatment of Miscanthus x giganteus using the ethanol organosolv process for ethanol production. Ind Eng Chem Res 48:8328–8334
Scordia D, Salvatore L, Thomas WJ (2013) Effectiveness of dilute oxalic acid pretreatment of Miscanthus × giganteus biomass for ethanol production. Biomass Bioenerg 59:540–548
Li H, Li C, Sang T, Xu J (2013) Pretreatment on Miscanthus lutarioriparious by liquid hot water for efficient ethanol production. Biotechnol Biofuels 6:76
Han M, Kim Y, Koo B, Choi G-W (2011) Bioethanol production by Miscanthus as a lignocellulosic biomass: focus on high efficiency conversion to glucose and ethanol. Bioresources 6(2):1939–1953
Acknowledgments
This study was financially supported by the Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea (no. 309016-05).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Han, M., Moon, SK. & Choi, GW. Pretreatment solution recycling and high-concentration output for economical production of bioethanol. Bioprocess Biosyst Eng 37, 2205–2213 (2014). https://doi.org/10.1007/s00449-014-1198-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-014-1198-1