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Production of bio-fuel ethanol from distilled grain waste eluted from Chinese spirit making process

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Abstract

Distilled grain waste eluted from Chinese spirit making is rich in carbohydrates, and could potentially serve as feedstock for the production of bio-fuel ethanol. Our study evaluated two types of saccharification methods that convert distilled grain waste to monosaccharides: enzymatic saccharification and concentrated H2SO4 saccharification. Results showed that enzymatic saccharification performed unsatisfactorily because of inefficient removal of lignin during pretreatment. Concentrated H2SO4 saccharification led to a total sugar recovery efficiency of 79.0 %, and to considerably higher sugar concentrations than enzymatic saccharification. The process of ethanol production from distilled grain waste based on concentrated H2SO4 saccharification was then studied. The process mainly consisted of concentrated H2SO4 saccharification, solid–liquid separation, decoloration, sugar–acid separation, oligosaccharide hydrolysis, and continuous ethanol fermentation. An improved simulated moving bed system was employed to separate sugars from acid after concentrated H2SO4 saccharification, by which 95.8 % of glucose and 85.8 % of xylose went into the sugar-rich fraction, while 83.3 % of H2SO4 went into the acid-rich fraction. A flocculating yeast strain, Saccharomyces cerevisiae KF-7, was used for continuous ethanol fermentation, which produced an ethanol yield of 91.9–98.9 %, based on glucose concentration.

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References

  1. Zhang J, Zhang WX, Wu ZY, Yang J, Liu YH, Zhong X, Deng Y (2008) A comparison of different dilute solution explosion pretreatment for conversion of distillers’ grains into ethanol. Prep Biochem Biotechnol 43:1–21

    Article  CAS  Google Scholar 

  2. Tan L, Liu YH, Zhang WX (2011) Study on two-stage dilute hydrochloric acid saccharification of fermented grain waste. Food Ferment Technol 47(25–27):31

    Google Scholar 

  3. Zhou XB, Zheng P (2013) Spirit-based distillers’ grain as a promising raw material for succinic acid production. Biotechnol Lett 35:679–684

    Article  CAS  Google Scholar 

  4. Yan SB, Li J, Chen XS, Wu JY, Wang PC, Ye JF, Yao JM (2011) Enzymatical hydrolysis of food waste and ethanol production from the hydrolyzate. Renew Energy 36:1259–1265

    Article  CAS  Google Scholar 

  5. Zhang J, Zhang WX, Li SZ, You L, Zhang C, Sun CZ, Liu XB (2013) A two-step fermentation of distillers’ grains using Trichoderma viride and Rhodopseudomonas palustris for fish feed. Bioprocess Biosyst Eng. doi:10.1007/s00449-013-0887-5

    Google Scholar 

  6. Yang B, Wyman CE (2008) Pretreatment: the key to unlocking low-cost cellulosic ethanol. Biofuels Bioprod Bioref 2:26–40

    Article  CAS  Google Scholar 

  7. Dagnino EP, Chamorro ER, Romano SD, Felissia FE, Area MC (2013) Optimization of the acid pretreatment of rice husks to obtain fermentable sugars for bioethanol productions. Ind Crop Prod 42:363–368

    Article  CAS  Google Scholar 

  8. Tan L, Tang YQ, Nishimura H, Takei S, Morimura S, Kida K (2013) Efficient production for bioethanol from corn stover by pretreatment with a combination of sulfuric acid and sodium hydroxide. Prep Biochem Biotechnol 43:682–695

    Article  CAS  Google Scholar 

  9. Laatikainen M, Heinonen J, Sainio T (2011) Modeling of chromatographic separation of concentrated-acid hydrolysates. Sep Purif Technol 80:610–619

    Article  CAS  Google Scholar 

  10. Liu ZS, Wu XL, Kida K, Tang YQ (2012) Corn stover saccharification with concentrated sulfuric acid: effects of saccharification conditions on sugar recovery and by-product generation. Bioresour Technol 119:224–233

    Article  CAS  Google Scholar 

  11. Sun ZY, Tang YQ, Iwanaga T, Sho T, Kida K (2011) Production of fuel ethanol from bamboo by concentrated sulfuric acid hydrolysis followed by continuous ethanol fermentation. Bioresour Technol 102:10929–10935

    Article  CAS  Google Scholar 

  12. Sun ZY, Tang YQ, Morimura S, Kida K (2013) Reduction in environmental impact of sulfuric acid hydrolysis of bamboo for production of fuel ethanol. Bioresour Technol 128:87–93

    Article  CAS  Google Scholar 

  13. Moe ST, Janga KK, Hertzberg T, Hagg MB, Oyaas K, Dyrset N (2012) Saccharification of lignocellulosic biomass for biofuel and biorefinery applications—a renaissance for the concentrated acid hydrolysis? Energy Procedia 2:50–58

    Article  Google Scholar 

  14. Adney B, Baker J (2008) Laboratory analytical procedure: Measurement of cellulase activites. National Renewable Energy Laboratory, Golden

    Google Scholar 

  15. Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597

    Article  Google Scholar 

  16. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2011) Laboratory analytical procedure: determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory, Golden

    Google Scholar 

  17. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Laboratory analytical procedure: Determination of ash in biomass. National Renewable Energy Laboratory, Golden

    Google Scholar 

  18. Kida K, Ikbal Sonoda Y, Kawase M, Nomura T (1991) Influence of mineral nutrients on high performance during anaerobic treatment of wastewater from a beer brewery. J Ferment Bioeng 72:54–57

    Article  CAS  Google Scholar 

  19. Tang YQ, An MZ, Liu K, Nagai S, Shigematsu T, Morimura S, Kida K (2006) Ethanol production from acid hydrolysate of wood biomass using the flocculating yeast Saccharomyces cerevisiae Strain KF-7. Process Biochem 41:909–914

    Article  CAS  Google Scholar 

  20. Cuvilas CA, Yang WH (2012) Spruce pretreatment for thermal application: water, alkali, and diluted acid hydrolysis. Energy Fuels 26:6426–6431

    Article  CAS  Google Scholar 

  21. Balat M (2011) Production of bioethanol form lignocellulosic materials via the biochemical pathway: a review. Energy Convers Manag 52:858–875

    Article  CAS  Google Scholar 

  22. Lynam JG, Reza MT, Vasquez VR, Coronella CJ (2012) Pretreatment of rice hulls by ionic liquid dissolution. Bioresour Technol 114:629–636

    Article  CAS  Google Scholar 

  23. Yang CY, Sheih IC, Fang TJ (2012) Fermentation of rice hull by Aspergillus japonicus under ultrasonic pretreatment. Ultrason Sonochem 19:687–691

    Article  CAS  Google Scholar 

  24. Chu CY, Wu SY, Tsai CY, Lin CY (2011) Kinetics of cotton cellulose hydrolysis using concentrated acid and fermentative hydrogen production from hydrolyzate. Int J Hydrog Energy 36:8743–8750

    Article  CAS  Google Scholar 

  25. Iranmahboob J, Nadim F, Monemi S (2002) Optimization acid-hydrolysis: a critical step for production of ethanol from mixed wood chips. Biomass Bioenerg 22:401–404

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto, Kumamoto, 860-8555, Japan

    Li Tan & Shigeru Morimura

  2. College of Architecture and Environment, Sichuan University, No. 24 South Section 1 First Ring Road, Chengdu, 610065, Sichuan, China

    Zhaoyong Sun, Yueqin Tang & Kenji Kida

  3. College of Light Industry, Textile and Food Engineering, Sichuan University, No. 24 South Section 1 First Ring Road, Chengdu, 610065, Sichuan, China

    Wenxue Zhang

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  1. Li Tan
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  2. Zhaoyong Sun
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  3. Wenxue Zhang
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  4. Yueqin Tang
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  5. Shigeru Morimura
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  6. Kenji Kida
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Correspondence to Kenji Kida.

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Tan, L., Sun, Z., Zhang, W. et al. Production of bio-fuel ethanol from distilled grain waste eluted from Chinese spirit making process. Bioprocess Biosyst Eng 37, 2031–2038 (2014). https://doi.org/10.1007/s00449-014-1178-5

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  • DOI: https://doi.org/10.1007/s00449-014-1178-5

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