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In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements

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Abstract

The need for bone repair has increased as the population ages. In this research, calcium phosphate cements, with and without chitosan (CS) and hyaluronic acid (HA), were synthesized. The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy. The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid. The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs). The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA. Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces. Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture. These results reveal the potential of the CPC–CS/HA composites to be used in bone tissue engineering.

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Acknowledgments

The authors would like to acknowledge the Iran National Science Foundation (INSF) for the financial support of this work through Grant No. 89001740.

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  1. Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Alborz, Iran

    Saeed Hesaraki & Nader Nezafati

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  1. Saeed Hesaraki
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  2. Nader Nezafati
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Correspondence to Nader Nezafati.

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Hesaraki, S., Nezafati, N. In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements. Bioprocess Biosyst Eng 37, 1507–1516 (2014). https://doi.org/10.1007/s00449-013-1122-0

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