Acetone–butanol–ethanol production from corn stover pretreated by alkaline twin-screw extrusion pretreatment
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In this study, the alkaline twin-screw extrusion pretreated corn stover was subjected to enzymatic hydrolysis after washing. The impact of solid loading and enzyme dose on enzymatic hydrolysis was investigated. It was found that 68.2 g/L of total fermentable sugar could be obtained after enzymatic hydrolysis with the solid loading of 10 %, while the highest sugar recovery of 91.07 % was achieved when the solid loading was 2 % with the cellulase dose of 24 FPU/g substrate. Subsequently, the hydrolyzate was fermented by Clostridium acetobutylicum ATCC 824. The acetone–butanol–ethanol (ABE) production of the hydrolyzate was compared with the glucose, xylose and simulated hydrolyzate medium which have the same reducing sugar concentration. It was shown that 7.1 g/L butanol and 11.2 g/L ABE could be produced after 72 h fermentation for the hydrolyzate obtained from enzymatic hydrolysis with 6 % solid loading. This is comparable to the glucose and simulated hydrozate medium, and the overall ABE yield could reach 0.112 g/g raw corn stover.
KeywordsAlkaline twin-screw extrusion pretreatment Enzymatic saccharification Acetone–butanol–ethanol (ABE) Corn stover Clostridium acetobutylicum ATCC 824 Fermentation
We are grateful for the support of the Natural Science Foundation of China and Shandong Province (No. 21206814, No. 21201174 and BS2009ZZ001), the SHELL Research Foundation as well as the National High Technology Research and Development Program (“863” program) of China (No. 2012AA022301).
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