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Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation

Bioprocess and Biosystems Engineering volume 37, pages 891–899 (2014)Cite this article

Abstract

This study investigated the effects of transformation conditions such as initial pH, the initial concentration of glucose and yeast extract in the medium, and the separate addition of ferulic acid and vanillic acid, on the production of vanillin through an analysis of competing by-product formation by Amycolatopsis sp. ATCC 39116. The extent and nature of by-product formation and vanillin yield were affected by initial pH and different initial concentrations of glucose and yeast extract in the medium, with a high yield of vanillin and high cell density obtained at pH 8.0, 10 g/l glucose, and 8 g/l yeast extract. High concentrations of ferulic acid were found to negatively affect cell density. Additional supplementation of 100 mg/l vanillic acid, a metabolically linked by-product, was found to result in a high concentration of vanillin and guaiacol, an intermediate of vanillin. Via an analysis of the effect of these transformation conditions on competing by-product formation, high concentrations of ferulic acid were transformed with a molar yield to vanillin of 96.1 and 95.2 %, by Amycolatopsis sp. ATCC 39116 and Streptomyces V1, respectively, together with a minor accumulation of by-products. These are among the highest performance values reported in the literature to date for Streptomyces in batch cultures.

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References

  1. Webster TM (1995) New perspectives on vanilla. Cereal Foods World 40(4):198–200

    Google Scholar 

  2. Priefert H, Rabenhorst J, Steinbuchel A (2001) Biotechnological production of vanillin. Appl Microbiol Biotechnol 56(3–4):296–314

    Article  CAS  Google Scholar 

  3. Toms A, Wood JM (1970) The degradation of trans-ferulic acid by Pseudomonas acidovorans. Biochemistry 9(2):337–343

    Article  CAS  Google Scholar 

  4. Ashengroph M, Nahvi I, Zarkesh-Esfahani H, Momenbeik F (2012) Conversion of isoeugenol to vanillin by Psychrobacter sp. strain CSW4. Appl Biochem Biotechnol 166(1):1–12

    Article  CAS  Google Scholar 

  5. Ashengroph M, Nahvi I, Zarkesh-Esfahani H, Momenbeik F (2011) Candida galli strain PGO6: a novel isolated yeast strain capable of transformation of isoeugenol into vanillin and vanillic acid. Curr Microbiol 62(3):990–998

    Article  CAS  Google Scholar 

  6. Ashengroph M, Nahvi I, Zarkesh-Esfahani H, Momenbeik F (2011) Pseudomonas resinovorans SPR1, a newly isolated strain with potential of transforming eugenol to vanillin and vanillic acid. New Biotechnol 28(6):656–664

    Article  CAS  Google Scholar 

  7. Su F, Hua DL, Zhang ZB, Wang XY, Tang HZ, Tao F, Tai C, Wu QL, Wu G, Xu P (2011) Genome sequence of bacillus pumilus S-1, an efficient isoeugenol-utilizing producer for natural vanillin. J Bacteriol 193(22):6400–6401

    Article  CAS  Google Scholar 

  8. Hua DL, Ma CQ, Song LF, Lin S, Zhang ZB, Deng ZX, Xu P (2007) Enhanced vanillin production from ferulic acid using adsorbent resin. Appl Microbiol Biotechnol 74(4):783–790

    Article  CAS  Google Scholar 

  9. Wangrangsimagul N, Klinsakul K, Vangnai AS, Wongkongkatep J, Inprakhon P, Honda K, Ohtake H, Kato J, Pongtharangkul T (2012) Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri 13. Appl Microbiol Biotechnol 93(2):555–563

    Article  CAS  Google Scholar 

  10. Ghosh S, Sachan A, Sen SK, Mitra A (2007) Microbial transformation of ferulic acid to vanillic acid by Streptomyces sannanensis MTCC 6637. J Ind Microbiol Biotechnol 34(2):131–138

    Article  CAS  Google Scholar 

  11. Narbad A, Gasson MJ (1998) Metabolism of ferulic acid via vanillin using a novel CoA-dependent pathway in a newly-isolated strain of Pseudomonas fluorescens. Microbiology 144(Pt 5):1397–1405

    Article  CAS  Google Scholar 

  12. Bode HB, Muller R (2003) Possibility of bacterial recruitment of plant genes associated with the biosynthesis of secondary metabolites. Plant Physiol 132(3):1153–1161

    Article  CAS  Google Scholar 

  13. Muheim A, Lerch K (1999) Towards a high-yield bioconversion of ferulic acid to vanillin. Appl Microbiol Biotechnol 51(4):456–461

    Article  CAS  Google Scholar 

  14. Zhao LQ, Sun ZH, Zheng P, Zhu LL (2005) Biotransformation of isoeugenol to vanillin by a novel strain of Bacillus fusiformis. Biotechnol Lett 27(19):1505–1509

    Article  CAS  Google Scholar 

  15. Mathew S, Abraham TE, Sudheesh S (2007) Rapid conversion of ferulic acid to 4-vinyl guaiacol and vanillin metabolites by Debaryomyces hansenii. J Mol Catal B-Enzymatic 44(2):48–52

    Article  CAS  Google Scholar 

  16. Mathew S, Abraham TE, Sudheesh S (2007) Rapid conversion of ferulic acid to 4-vinyl guaiacol and vanillin metabolites by Debaryomyces hansenii. J Mol Catal B Enzym 44(2):48–52

    Article  CAS  Google Scholar 

  17. Karmakar B, Vohra RM, Nandanwar H, Sharma P, Gupta KG, Sobti RC (2000) Rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of Bacillus coagulans. J Biotechnol 80:195–202

    Article  CAS  Google Scholar 

  18. Fleige C, Hansen G, Kroll J, Steinbuchel A (2013) Investigation of the Amycolatopsis sp. strain ATCC 39116 vanillin dehydrogenase and its impact on the biotechnical production of vanillin. Appl Environ Microbiol 79(1):81–90

    Article  CAS  Google Scholar 

  19. Sutherland JB, Crawford DL, Pometto AL III (1983) Metabolism of cinnamic, p-coumaric, and ferulic acids by Streptomyces setonii. Can J Microbiol 29(10):1253–1257

    Article  CAS  Google Scholar 

  20. Li T, Rosazza JP (2000) Biocatalytic synthesis of vanillin. Appl Environ Microbiol 66(2):684–687

    Article  CAS  Google Scholar 

  21. Yoon SH, Lee EG, Das A, Lee SH, Li C, Ryu HK, Choi MS, Seo WT, Kim SW (2007) Enhanced vanillin production from recombinant E-coli using NTG mutagenesis and adsorbent resin. Biotechnol Prog 23(5):1143–1148

    Google Scholar 

  22. Gao F, Daugulis AJ (2009) Bioproduction of the aroma compound 2-phenylethanol in a solid-liquid two-phase partitioning bioreactor system by kluyveromyces marxianus. Biotechnol Bioeng 104(2):332–339

    Article  CAS  Google Scholar 

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Acknowledgments

The Project was supported by Social Development and Scientific and Technological Plan in Shaanxi Province of China (Program No. 693102), Science and technology projects in Xi’an of China (No.694180) and the Fundamental Research Funds for the Central Universities of China (No.GK201003002). Many thanks to Julian Dafoe and Eric Peterson for their support during this study.

Conflict of interest

The authors declare that they have no conflict of interest.

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Affiliations

  1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Shaanxi Normal University, 199 Chang’an South Road, Xi’an, 710055, Shaanxi, People’s Republic of China

    Xiao-kui Ma

  2. Department of Chemical Engineering, Queen’s University, Kingston, ON, K7L 3N6, Canada

    Andrew J. Daugulis

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  1. Xiao-kui Ma
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  2. Andrew J. Daugulis
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Correspondence to Andrew J. Daugulis.

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Ma, Xk., Daugulis, A.J. Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation. Bioprocess Biosyst Eng 37, 891–899 (2014). https://doi.org/10.1007/s00449-013-1060-x

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  • DOI: https://doi.org/10.1007/s00449-013-1060-x

Keywords

  • Transformation condition
  • Vanillin production
  • Amycolatopsis sp. ATCC 39116
  • An analysis of competing by-product formation
  • Streptomyces V1