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Physiology of Lichtheimia ramosa obtained by solid-state bioprocess using fruit wastes as substrate

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Abstract

Due to the amount of nutrients available in the agroindustrial wastes, these can be converted into high added-value products by the action of microorganisms in solid-state bioprocesses. The aim of this work was to evaluate the growth physiology and lipase production of the fungus Lichtheimia ramosa using the following Brazilian savannah fruit wastes as substrates: bocaiuva (Acrocomia aculeata), pequi (Caryocar brasiliense), guavira (Campomanesia pubescens), araticum (Annona crassiflora) and seriguela (Spondias purpurea). These residues were triturated, homogenized, adjusted to pH 5.0 and 60 % moisture, sterilized and packaged in plastic tray-type bioreactors before inoculation with 10 % (w/v) of L. ramosa pre-culture medium. The cultivations were conducted in a bacteriological incubator at 30 °C for 40 days. Samples were taken every 5 days and fungi and bacteria contents, proximate composition and lipase activity were evaluated. The maximum fungal counting was observed between 25 and 35 days. L. ramosa reached the stationary phase next to 40 days in all substrates. Mesophilic and psicrophilic aerobic bacteria were not detected. Protein enrichment was obtained for all media, being superior in seriguela residues (391.66 %), followed by pequi (160.04 %), araticum (143.31 %), guavira (102.42 %), and bocaiuva (67.88 %). Lipase production was observed in all cultivated media, except in pequi residues that showed decreasing lipase activity. The higher production was observed in guavira (1.12 U/g) followed by araticum (0.58 U/g), seriguela (0.41 U/g) and bocaiuva (0.21 U/g) waste substrates. It was concluded that the studied fruit wastes have been successfully utilized as substrates for protein enrichment and lipase production with L. ramosa.

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Acknowledgments

The authors acknowledge the financial support provided by the Scientific and Technological Research National Council of Brazil (CNPq).

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Authors and Affiliations

  1. Laboratory of Bioengineering, Faculty of Engineering, Federal University of Grande Dourados, Dourados, MS, CEP 79804-970, Brazil

    Cinthia Aparecida de Andrade Silva, Maria Priscila Franco Lacerda & Gustavo Graciano Fonseca

  2. Laboratory of Enzymology, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil

    Rodrigo Simões Ribeiro Leite

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  1. Cinthia Aparecida de Andrade Silva
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  2. Maria Priscila Franco Lacerda
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Correspondence to Gustavo Graciano Fonseca.

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de Andrade Silva, C.A., Lacerda, M.P.F., Leite, R.S.R. et al. Physiology of Lichtheimia ramosa obtained by solid-state bioprocess using fruit wastes as substrate. Bioprocess Biosyst Eng 37, 727–734 (2014). https://doi.org/10.1007/s00449-013-1043-y

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