Abstract
We developed a localized surface plasmon resonance (LSPR)-based label-free optical biosensor for detection of salbutamol (Sal). Hollow gold nanoparticles (HGNs) which deposited on transparent indium tin oxide (ITO) film coated glass was used to sensing platform. Antibody against Sal was immobilized on HGN surface to recognize the target Sal molecules. Thus, the change of LSPR peak was proportional to the concentration of Sal in the solution. The experimental results demonstrated that the LSPR immunosensor possessed a good sensitivity and a high selectivity for Sal. The detection range for Sal was from 0.05 to 0.8 μg/mL with a correlation coefficient of 0.996. The biosensor was applied for the detection for Sal in spiked animal feed and pork liver samples, and the recoveries were in the range of 97–105 %. Therefore, it is expected that this approach may offer a new method in designing label-free LSPR immunosensor for detection of small molecules.
Similar content being viewed by others
References
Mayer KM, Hafner JH (2011) Localized surface plasmon resonance sensors. Chem Rev 111:3828–3857
Stewart ME, Anderton CR, Thompson LB, Maria J, Gray SK, Rogers JA, Nuzzo RG (2008) Nanostructured plasmonic sensors. Chem Rev 108:494–521
Chen H, Ming T, Zhao L, Wang F, Sun L, Wang J, Yan C (2010) Plasmon—molecule interactions. Nano Today 5:494–505
Sepúlveda B, Angelomé PC, Lechuga LM, Liz-Marzán LM (2009) LSPR-based nanobiosensors. Nano Today 4:244–251
Shon Y, Choi HY, Guerrero MS, Kwon C (2009) Preparation of nanostructured film arrays for transmission localized surface plasmon sensing. Plasmonics 4:95–105
Endo T, Kerman K, Nagatani N, Takamura Y, Tamiya E (2005) Label-free detection of peptide nucleic acid-DNA hybridization using localized surface plasmon resonance based optical biosensor. Anal Chem 77:6976–6984
Nath N, Chilkoti A (2004) Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. Anal Chem 76:5370–5378
Li C, Wu C, Zheng J, Lai J, Zhang C, Zhao Y (2010) LSPR sensing of molecular biothiols based on noncoupled gold nanorods. Langmuir 26:9130–9135
Vestergaard M, Kerman K, Kim D, Hiep HM, Tamiya E (2008) Detection of Alzheimer’s tau protein using localized surface plasmon resonance-based immunochip. Talanta 74:1038–1042
Wang C, Ma Z, Wang T, Su Z (2006) Synthesis, assembly, and biofunctionalization of silica-coated gold nanorods for colorimetric biosensing. Adv Funct Mater 16:1673–1678
Mayer KM, Lee S, Liao H, Rostro BC, Fuentes A, Scully PT, Nehl CL, Hafner JH (2008) A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods. ACS Nano 2:687–692
Fan M, Thompson M, Andrade ML, Brolo AG (2010) Silver nanoparticles on a plastic platform for localized surface plasmon resonance biosensing. Anal Chem 82:350–6352
Miller MM, Lazarides AA (2005) Sensitivity of metal nanoparticle surface plasmon resonance to the dielectric environment. J Phys Chem B 109:21556–21565
Wan D, Chen H, Lin Y, Chuang S, Shieh J, Chen S (2009) Using spectroscopic ellipsometry to characterize and apply the optical constants of hollow gold nanoparticles. ACS Nano 3:960–970
Vongsavat V, Vittur BM, Bryan WW, Kim J, Lee TR (2011) Ultrasmall hollow gold_silver nanoshells with extinctions strongly red-shifted to the near-infrared. ACS Appl Mater Interfaces 3:3616–3624
Sun Y, Xia Y (2002) Increased sensitivity of surface plasmon resonance of gold nanoshells compared to that of gold solid colloids in response to environmental changes. Anal Chem 74:5297–5305
Wang Y, Qian W, Tan Y, Ding S (2008) A label-free biosensor based on gold nanoshell monolayers for monitoring biomolecular interactions in diluted whole blood. Biosens Bioelectron 23:1166–1170
Hu T, Lin Y, Yan J, Di J (2013) Synthesis of hollow gold nanoparticles on the surface of indium tin oxide glass and their application for plasmonic biosensor. Spectrochim Acta A Mol Biomol Spectrosc 110:72–77
Sheu S, Lei Y, Tai Y, Chang T, Kuo T (2009) Screening of salbutamol residues in swine meat and animal feed by an enzyme immunoassay in Taiwan. Anal Chim Acta 654:148–153
Libretto SE (1994) A review of the toxicology of salbutamol (albuterol). Arch Toxicol 68:213–216
Chai C, Liu G, Li F, Liu X, Yao B, Wang L (2010) Towards the development of a portable sensor based on a molecularly imprinted membrane for the rapid determination of salbutamol in pig urine. Anal Chim Acta 675:185–190
Mohamed GG, Khalil SM, Zayed MA, El-Shall MAE (2002) 2,6-Dichloroquinone chlorimide and 7,7,8,8-tetracyanoquinodimethane reagents for the spectrophotometric determination of salbutamol in pure and dosage forms. J Pharmaceut Biomed 28:1127–1133
Basavaiah K, Prameela HC (2003) Three useful bromimetric methods for the determination of salbutamol sulfate. Anal Bioanal Chem 376:879–883
Mazhara SHRA, Chrystyn H (2009) New HPLC assay for urinary salbutamol concentrations in samples collected post-inhalation. J Pharmaceut Biomed 50:175–182
Zhang J, Xu Y, Di X, Wu M (2006) Quantitation of salbutamol in human urine by liquid chromatography–electrospray ionization mass spectrometry. J Chromatogr B 831:328–332
Mikuš P, Valášková I, Havráánek E (2005) Determination of salbutamol in pharmaceuticals by capillary electrophoresis. Arch Pharm Chem Life Sci 338:498–501
Lindino CA, Bulhões LOS (2007) Determination of fenoterol and salbutamol in pharmaceutical formulations by electrogenerated chemiluminescence. Talanta 72:1746–1751
Goyal RN, Kaur D, Singh SP, Pandey AK (2008) Effect of graphite and metallic impurities of C60 fullerene on determination of salbutamol in biological fluids. Talanta 75:63–69
Huang J, Lin Q, Zhang X, He X, Xing X, Lian W, Zuo M, Zhang Q (2011) Electrochemical immunosensor based on polyaniline/poly (acrylic acid) and Au-hybrid graphene nanocomposite for sensitivity enhanced detection of salbutamol. Food Res Int 44:92–97
Deng J, Song Y, Wang Y, Di J (2010) Label-free optical biosensor based on localized surface plasmon resonance of twin-linked gold nanoparticles electrodeposited on ITO glass. Biosens Bioelectron 26:615–619
Marinakos SM, Chen S, Chilkoti A (2007) Plasmonic detection of a model analyte in serum by a gold nanorod. Anal Chem 79:5278–5283
Acknowledgments
This work was financially supported by the National Natural Science Foundation of China (No. 21075086), the Project of Scientific and Technologic Infrastructure of Suzhou (SZS201207) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yan, Z., Hu, T., Guo, W. et al. A label-free immunosensor for determination of salbutamol based on localized surface plasmon resonance biosensing. Bioprocess Biosyst Eng 37, 651–657 (2014). https://doi.org/10.1007/s00449-013-1034-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-013-1034-z