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Enhanced gellan gum production by hydrogen peroxide (H2O2) induced oxidative stresses in Sphingomonas paucimobilis

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Abstract

In this study, the effect of H2O2-induced oxidative stress on gellan gum production and cell growth were investigated. Gellan gum production was improved and cell growth was inhibited by H2O2. A multiple H2O2 stresses with different concentrations were developed to optimize gellan gum production. A maximal gellan gum yield (22.52 g/L), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/L H2O2 added at 6, 12, 18, 24 h, respectively. Moreover, UDP-glucose pyrophosphorylase activity and glucosyltransferase activity were increased with H2O2 stresses. This new strategy of multiple H2O2-induced oxidative stresses would be further applied to gellan gum production in future study.

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Acknowledgments

The authors gratefully acknowledge the financial support from The provincial natural science research project of China (No. KJ2013B215).

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Authors and Affiliations

  1. Department of Life Science, Hefei Normal University, Hefei, AnHui, 230061, China

    Guilan Zhu

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Guilan Zhu, Long Sheng & Qunyi Tong

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  1. Guilan Zhu
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  2. Long Sheng
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Correspondence to Guilan Zhu.

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Zhu, G., Sheng, L. & Tong, Q. Enhanced gellan gum production by hydrogen peroxide (H2O2) induced oxidative stresses in Sphingomonas paucimobilis . Bioprocess Biosyst Eng 37, 743–748 (2014). https://doi.org/10.1007/s00449-013-1030-3

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  • DOI: https://doi.org/10.1007/s00449-013-1030-3

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