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Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold

Bioprocess and Biosystems Engineering volume 37, pages 433–440 (2014)Cite this article

An Erratum to this article was published on 22 October 2014

Abstract

In bone tissue engineering, porous hydroxyapatite (HAp) is used as filling material for bone defects, augmentation, artificial bone graft and scaffold material. The present paper compares the preparation and characterization of HAp from fish scale (FS) and synthetic body fluid (SBF) solution. Thermo gravimetric analysis, differential thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and particle size analysis of the samples have been performed. The analysis indicates that synthesized HAp consists of sub-micron HAp particle with Ca/P ratio corresponding to FS and SBF 1.62 and 1.71, respectively. MTT assay and quantitative DNA analysis show growth and proliferation of cells over the HA scaffold with the increase in time. The shape and size (morphology) of mesenchymal stem cells after 3 days show a transition from rounded shape to elongated and flattened shape expressing its spreading behavior. These results confirm that HAp bio-materials from fish scale are physico-chemically and biologically equivalent to the chemically synthesized HAp from SBF. Biological HAp, thus, possesses a great potential for conversion of industrial by-product into highly valuable compounds using simple effective and novel processes.

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Affiliations

  1. Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, 769 008, Odisha, India

    Niladri Nath Panda & Krishna Pramanik

  2. Institute of Minerals and Materials Technology (CSIR), Bhubaneswar, 751 013, India

    Lala Behari Sukla

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  1. Niladri Nath Panda
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  2. Krishna Pramanik
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  3. Lala Behari Sukla
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Correspondence to Lala Behari Sukla.

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Panda, N.N., Pramanik, K. & Sukla, L.B. Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold. Bioprocess Biosyst Eng 37, 433–440 (2014). https://doi.org/10.1007/s00449-013-1009-0

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  • DOI: https://doi.org/10.1007/s00449-013-1009-0

Keywords

  • Hydroxyapatite
  • Biomaterials
  • Bio implants
  • Cytotoxicity