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Analysis of the characteristics of short-cut nitrifying granular sludge and pollutant removal processes in a sequencing batch reactor

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Abstract

Aerobic granular sludge is a new type of microbe auto-immobilization technology; in this paper, short-cut nitrification and denitrification were effectively combined with the granular sludge technology. Simultaneous nitrification and denitrification granules were developed in a sequencing batch reactor (SBR) using synthetic wastewater with a high concentration of ammonia nitrogen at 25 °C with a dissolved oxygen concentration above 2.0 mg/L and a 15 days sludge retention time. The characteristics of the sludge and the removal efficiency were studied, and the removal mechanisms of the pollutants and the process of short-cut nitrification were analyzed. The average granule diameter of the granular sludge was 704.0 μm. The removal rates of pollutants and the accumulation rate of nitrite in the SBR were studied. During treatment of wastewater with a high concentration of ammonia nitrogen, simultaneous nitrification, and denitrification and the stripping process could contribute to the removal of total nitrogen. The high pH value, the high concentration of free ammonia, and the delamination of granular sludge were the main factors contributing to the short-cut nitrification property of granular sludge in the reaction process.

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References

  1. Thanh BX, Visvanathan C, Aim RB (2009) Characterization of aerobic granular sludge at various organic loading rates. Process Biochem 44(2):242–245

    Article  CAS  Google Scholar 

  2. Gao DW, Yuan XJ, Liang H (2012) Reactivation performance of aerobic granules under different storage strategies. Water Res 46:3315–3322

    Article  CAS  Google Scholar 

  3. Wang JL, Zhang ZJ, Wu WW (2009) Research advances in aerobic granular sludge. Acta Scientiae Circumstantiae 29(3):449–473 (in Chinese)

    Google Scholar 

  4. Coma M, Verawaty M, Pijuan M, Yuan Z, Bond PL (2012) Enhancing aerobic granulation for biological nutrient removal from domestic wastewater. Bioresour Technol 103:101–108

    Article  CAS  Google Scholar 

  5. Su KZ, Yu HQ (2005) Formation and characterization of aerobic granules in a sequencing batch reactor treating soy-bean processing wastewater. Environ Sci Technol 39:2818–2827

    Article  CAS  Google Scholar 

  6. Wu CY, Peng YZ, Wang RD, Zhou YX (2012) Understanding the granulation process of activated sludge in a biological phosphorus removal sequencing batch reactor. Chemosphere 86(8):767–773

    Article  CAS  Google Scholar 

  7. Weber SD, Ludwig W, Schleifer KH, Schleifer JFried (2007) Microbial composition and structure of aerobic granular sewage biofilms. Appl Environ Microbiol 73:6233–6240

    Article  CAS  Google Scholar 

  8. Wu L, Peng C, Peng YZ, Li LY, Wang SY, Ma Y (2012) Effect of wastewater COD/N ratio on aerobic nitrifying sludge granulation and microbial population shift. J Environ Sci 24(2):234–241

    Article  CAS  Google Scholar 

  9. Lv Yi, Wan Chunli, Liu Xiang, Zhang Yi, Lee Duu-Jong, Tay Joo-Hwa (2013) Drying and re-cultivation of aerobic granules. Bioresour Technol 129:700–703

    Article  CAS  Google Scholar 

  10. Liu Y, Yang SF, Qin L, Tay JH (2004) A thermodynamic interpretation of cell hydrophobic in aerobic granulation. Appl Microbiol Biotechnol 64(3):410–415

    Article  CAS  Google Scholar 

  11. Liu Y, Yang SF, Tay JH, Liu QS, Qin L, Li Y (2004) Cell hydrophobicity is a triggering force of biogranulation. Enzym Microbial Technol 34(5):371–379

    Article  CAS  Google Scholar 

  12. Liu YQ, Liu Y, Tay JH (2004) The effects of extracellular polymeric substances on the formation and stability of biogranules. Appl Microbiol Biotechnol 65(2):143–148

    Article  CAS  Google Scholar 

  13. McSwain BS, Irvine RI, Hausner M et al (2005) Composition and distribution of extracellular polymeric substances in aerobic flocs and granular sludge. Appl Environ Microbiol 71(2):1051–1057

    Article  CAS  Google Scholar 

  14. Basheer F, Farooqi IH (2012) Biodegradation of p-cresol by aerobic granules in sequencing batch reactor. J Environ Sci 24(11):2012–2018

    Article  CAS  Google Scholar 

  15. Wang XH, Jiang X, Shi YJ, Gao MM, Yang S, Wang SG (2012) Effects of step-feed on granulation processes and nitrogen removal performances of partial nitrifying granules. Bioresour Technol 123:375–381

    Article  CAS  Google Scholar 

  16. Chen FY, Liu YQ, Tay JH, Ning P (2011) Operational strategies for nitrogen removal in granular sequencing batch reactor. J Hazard Mater 189:342–348

    Article  CAS  Google Scholar 

  17. Othman I, Anuar AN, Ujang Z, Hasyimah NR, Harun H, Chelliapan S (2013) Livestock wastewater treatment using aerobic granular sludge. Bioresour Technol 133:630–634

    Article  CAS  Google Scholar 

  18. Tay JH, Yang SF, Liu Y (2002) Hydraulic selection pressure-induced nitrifying granulation in sequencing batch reactors. Appl Microbiol Biotechnol 59:332–337

    Article  CAS  Google Scholar 

  19. Li AJ, Li XY, Yu HQ (2013) Aerobic sludge granulation facilitated by activated carbon for partial nitrification treatment of ammonia-rich wastewater. Chem Eng J 218:253–259

    Article  CAS  Google Scholar 

  20. Gao JF (2007) Effects of settling time and biofilm on the cultivation of nitrifying aerobic granular sludge. Environ Sci 28(6):1245–1251 (in Chinese)

    CAS  Google Scholar 

  21. Tsuneda S, Nagano T, Hoshino T, Ejiri Y, Noda N (2003) Characterization of nitrifying granules produced in an aerobic up flow fluidized bed reactor. Water Res 37:4965–4973

    Article  CAS  Google Scholar 

  22. Belmonte M, Vázquez-Padín JR, Figueroa M, Francob A, Mosquera-Corralb A, Camposb JL, Méndezb R (2009) Characteristics of nitrifying granules developed in an air pulsing SBR. Process Biochem 44:602–606

    Article  CAS  Google Scholar 

  23. Liu Y, Yang SF, Tay JH (2004) Improved stability of aerobic granules by selecting slow-growing nitrifying bacteria. J Biotechnol 108:161–169

    Article  CAS  Google Scholar 

  24. Zhong C, Wang YQ, Wang YJ, Lv JP, Li YC, Zhu JR (2013) High-ratenitrogen removal and its behavior of granular sequence batch Reactor under step-feed operational strategy. Bio Resour Technol 134:101–106

    Article  CAS  Google Scholar 

  25. Song YJ, Ishii S, Rathnayake L, Tsukasa I, Satoh H, Okabe S (2013) Development and characterization of the partial nitrification aerobic granules in a sequencing batch airlift reactor. http://dx.doi.org/10.1016/j.biortech.2013.04.018

  26. Aslan S, Miller L, Dahab M (2009) Ammonium oxidation via nitrite accumulation under limited oxygen concentration in sequencing batch reactors. Bio Resour Technol 100:659–664

    Article  CAS  Google Scholar 

  27. APHA (1992) Standard methods for the examination of water and wastewater·18th ed·washington DC: American Public Health Association[M], America Water Works Association and Water Environment Federation

  28. Anthonisen AC, Loehr RC, Prakasam TBS, Srinath EG (1976) Inhibition of nitrification by ammonia and nitrous acid. J Water Pollut Control Federation 48(5):835–852

    CAS  Google Scholar 

  29. Wu L, Peng YZ, Wang SY (2010) Quick start and maintenance of nitrifying granular sludge in SBR process. CIESC J 11(11):2931–2937 (in Chinese)

    Google Scholar 

  30. Gao JF, Guo JQ, Chen RN, Su K, Peng YZ (2008) Influence of SBR drainage height and diameter ratio on aerobic sludge granulation. China Environ Sci 28(6):512–516

    CAS  Google Scholar 

  31. L iu YQ, Tay JH (2007) In flu ence of cycle t im e on k inetic beh aviors of steady-state aerob ic granu les in sequen cing batch reactors. Enzym Microbial Technol 41(4):516–522

    Article  CAS  Google Scholar 

  32. Shi XY, Yu HQ, Sun YJ, Huang X (2009) Characteristics of aerobic granules rich in autotrophic ammonium-oxidizing bacteria in a sequencing batch reactor. Chem Eng J 47:102–109

    Article  Google Scholar 

  33. Wu L, Peng CY, Peng YZ, Li LY, Wang Shuying, Ma Yong (2012) Effect of wastewater COD/N ratio on aerobic nitrifying sludge granulation and microbial population shift. J Environ Sci 24(2):234–241

    Article  CAS  Google Scholar 

  34. Li AJ, Yang SF, Li XY, Gu JD (2008) Microbial population dynamics during aerobic sludge granulation at different organic loading rates. Water Res 42:3552–3560

    Article  CAS  Google Scholar 

  35. Bassin JP, Pronk M, Kraan R (2011) Ammonium adsorption in aerobic granular sludge, activated Sludge and anammox granules. Water Res 45:5257–5265

    Article  CAS  Google Scholar 

  36. Pochana K, Keller J (1999) Study of factors affecting simultaneous nitrification and denitrification (SND). Water Sci Technol 39(6):61–68

    Article  CAS  Google Scholar 

  37. Fang S, Li XH (2001) The effect of pH on partial nitrification-denitrification rate of high ammonia-containing wastewater. J Chem Eng Chin Univ 15(4):346–350 (in Chinese)

    CAS  Google Scholar 

  38. Tokutomi T (2004) Operation of a nitrite-type airlift reactor at low DO concentration. Water Sci Technol 49(5–6):81–88

    CAS  Google Scholar 

  39. Jenicek P, Svehla P, Zabranska J, Dohanyos M (2004) Factors affecting nitrogen removal by nitritation/denitritation. Water Sci Technol 9(5–6):73–79

    Google Scholar 

  40. Yang SF, Tay JH, Liu Yu (2004) Inhibition of free ammonia to the formation of aerobic granules. Biochem Eng J 1:41–48

    Article  Google Scholar 

  41. Hellinga C, Schellen AAJC, Mulder JW, van Loosdrecht MCM, Heijnen JJ (1998) The SHARON process: an innovative method for nitrogen removal from ammonium-rich wastewater. Wat Sci Technol 37:135–142

    Google Scholar 

  42. Chang JS, Cha GC, Kim DJ (2002) Nitrite accumulation characteristics in the nitrification of high strength ammonia wastewater with biofilm airlift suspension reactor. J Korean Inst Chem Eng 40:114–120

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by National Natural Science Foundation for Young Scholars of China (NO. 51208084); Youth Foundation of Heilongjiang Province of China (NO. QC2012C089); Science and Technology Research Projects of Education Department of Heilongjiang Province of China (NO. 12521006); Heilong Jiang Postdoctoral Funds for scientific research initiation of China (NO. LBH-Q11166); National Science and Technology Pillar Program of China (NO. 2013BAJ12B02-4).

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Correspondence to Zhang Ying.

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Li-long, Y., Yu, L., Yuan, R. et al. Analysis of the characteristics of short-cut nitrifying granular sludge and pollutant removal processes in a sequencing batch reactor. Bioprocess Biosyst Eng 37, 125–132 (2014). https://doi.org/10.1007/s00449-013-1006-3

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  • DOI: https://doi.org/10.1007/s00449-013-1006-3

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