Abstract
d-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert d-galactose into d-galactonate, a valuable compound in the polymer and cosmetic industries. d-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L−1 d-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L−1 d-galactonate. Inherent metabolic pathways for assimilating both d-galactose and d-galactonate were blocked to enhance the production of d-galactonate. This approach finally led to a 7.3-fold increase with d-galactonate concentration of 1.24 g L−1 and yield of 62.0 %. Batch fermentation in 20 g L−1 d-galactose of E. coli ∆galK∆dgoK mutant expressing the gld resulted in 17.6 g L−1 of d-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of d-galactonate.
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This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0006693).
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H. Liu, K. R. M. Ramos and K. N. G. Valdehuesa equally contributed to this work.
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Liu, H., Ramos, K.R.M., Valdehuesa, K.N.G. et al. Metabolic engineering of Escherichia coli for biosynthesis of d-galactonate. Bioprocess Biosyst Eng 37, 383–391 (2014). https://doi.org/10.1007/s00449-013-1003-6
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DOI: https://doi.org/10.1007/s00449-013-1003-6