Bioprocess and Biosystems Engineering

, Volume 37, Issue 2, pp 107–114 | Cite as

Selective fengycin production in a modified rotating discs bioreactor

  • Omar Chtioui
  • Krasimir Dimitrov
  • Frédérique Gancel
  • Pascal Dhulster
  • Iordan Nikov
Original Paper

Abstract

Production of lipopeptides fengycin and surfactin in rotating discs bioreactor was studied. The effects of rotation velocity and the addition of agitators between the discs on volumetric oxygen transfer coefficient kLa were firstly studied in model media. Then the production of lipopeptides was also studied at different agitation conditions in the modified bioreactor (with agitators). The effect of agitation on dissolved oxygen, on submerged and immobilized biomass, on lipopeptide concentrations and yields and on the selectivity of the bioreaction was elucidated and discussed. The proposed modified rotating discs bioreactor allowed to obtain high fengycin concentrations (up to 787 mg L−1), but also better selectivity of the bioreaction towards fengycin (up to 88 %) and better yields of fengycin per glucose (up to 62.9 mg g−1), lipopeptides per glucose (up to 71.5 mg g−1), fengycin per biomass (up to 309 mg g−1) and lipopeptides per biomass (up to 396 mg g−1) than those reported in the literature. Highest fengycin production and selectivity were obtained at agitation velocity of 30 min−1. The proposed non-foaming fermentation process could contribute to the scale-up of lipopeptide fermentors and promote the industrial production of fengycin. The proposed bioreactor and bioprocess could be very useful also for the production of other molecules using bioprocesses requiring bubbleless oxygen supply.

Keywords

Fengycin Rotating discs bioreactor Lipopeptides Oxygen transfer B. subtilis ATCC 21332 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Omar Chtioui
    • 1
  • Krasimir Dimitrov
    • 1
  • Frédérique Gancel
    • 1
  • Pascal Dhulster
    • 1
  • Iordan Nikov
    • 1
  1. 1.Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech’Lille/IUT AUniversité Lille Nord de FranceVilleneuve d’Ascq CedexFrance

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