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High-level extracellular production of d-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach

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Abstract

The aim of this study is to achieve high-level extracellular production of d-Psicose-3-epimerase (DPE) with recombinant Escherichia coli. High-level production of DPE is one of the key factors in d-Psicose production. In the present study, the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 was modified by artificial synthesis for overexpression in E. coli. The total DPE activity reached 3.96 U mL−1 after optimization of the media composition, induction temperature, and concentration of inducer. Furthermore, it was found that addition of glycine had a positive effect on the extracellular production of DPE, which reached 3.5 U mL−1. Finally, a two-stage glycerol feeding strategy based on both the specific growth rate before induction and the amount of glycerol residues after induction was applied in a 3-L fermenter. After a series of optimal strategies in the 3-L fermenter, the total and extracellular DPE activity were 5.08- and 3.11-fold higher than that noted in the shake flask. The extracellular and intracellular DPE activity reached 10.9 and 13.2 U mL−1, achieving 25.5 and 31.1 % conversion of d-fructose to d-psicose, respectively. The systemic strategies presented in this study provide valuable novel information for the industrial application of DPE.

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Acknowledgments

This project was financially supported by the National High Technology Research and Development Program of China (863 Program, 2011AA100905), the National Natural Science Foundation of China (No. 30900013), the National Key Technology R&D Program in the 12th Five year Plan of China (2011BAK10B03), and the National High Technology Research and Development Program of China (863 Program, 2011AA100901).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Lei Gu, Juan Zhang & Baihong Liu

  2. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Guocheng Du

  3. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Jian Chen

  4. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China

    Lei Gu, Juan Zhang, Baihong Liu, Guocheng Du & Jian Chen

  5. College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, 610065, China

    Chongde Wu

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  1. Lei Gu
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  2. Juan Zhang
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  3. Baihong Liu
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  4. Chongde Wu
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Correspondence to Juan Zhang or Jian Chen.

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Gu, L., Zhang, J., Liu, B. et al. High-level extracellular production of d-Psicose-3-epimerase with recombinant Escherichia coli by a two-stage glycerol feeding approach. Bioprocess Biosyst Eng 36, 1767–1777 (2013). https://doi.org/10.1007/s00449-013-0952-0

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  • DOI: https://doi.org/10.1007/s00449-013-0952-0

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