Skip to main content
SpringerLink
Log in

Continuous production of high-purity fructooligosaccharides and ethanol by immobilized Aspergillus japonicus and Pichia heimii

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

High-purity fructooligosaccharides (FOS) were produced from sucrose by an innovative process incorporating immobilized Aspergillus japonicus and Pichia heimii cells. Intracellular FTase of A. japonicus converted sucrose into FOS and glucose, and P. heimii fermented glucose mainly into ethanol. The continuous production of FOS was carried out using a tanks-in-series bioreactor consisting of three stirred tanks. When a solution composed of 1 g L−1 yeast extract and 300 g L−1 sucrose was fed continuously to the bioreactor at a dilution rate of 0.1 h−1, FOS at a purity of up to 98.2 % could be achieved and the value-added byproduct ethanol at 79.6 g L−1 was also obtained. One gram of sucrose yielded 0.62 g FOS and 0.27 g ethanol. This immobilized dual-cell system was effective for continuous production of high-purity FOS and ethanol for as long as 10 days.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

F:

Fructose

G:

Glucose

GF:

Sucrose

GF2 :

1-Kestose

GF3 :

Nystose

GF4 :

1F-Fructosyl nystose

DP of FOS:

Degree of polymerization of FOS

Yc of FOS:

Conversion yield of FOS (g FOS produced/g sucrose consumed)

References

  1. Yun JW (1996) Fructooligosaccharides—occurrence, preparation, and application. Enzyme Microb Technol 19:107–117

    Article  CAS  Google Scholar 

  2. Sangeetha PT, Ramesh MN, Prapulla SG (2005) Recent trends in the microbial production, analysis and application of fructooligosaccharides. Trends Food Sci Technol 16:442–457

    Article  CAS  Google Scholar 

  3. Zafar TA, Weaver CM, Zhao Y, Martin BR, Wastney ME (2004) Nondigestible oligosaccharides increase calcium absorption and suppress bone resorption in ovariectomized rats. J Nutr 134:399–402

    CAS  Google Scholar 

  4. Ooi LG, Liong MT (2010) Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. Int J Mol Sci 5:37–50

    Google Scholar 

  5. Monsan PM, Ouarné F (2010) Oligosaccharides derived from sucrose. In: Charalampopoulos D, Rastall A (eds) The prebiotics and probiotics science and technology. Springer, Berlin

    Google Scholar 

  6. Duan KJ, Chen JS, Sheu DC (1994) Kinetic studies and mathematical model for enzymatic production of fructooligosaccharides from sucrose. Enzyme Microb Technol 16:334–339

    Article  CAS  Google Scholar 

  7. Jung KH, Kim JH, Jeon YJ, Lee JH (1993) Production of high fructo-oligosaccharide syrup with two enzyme system of fructosyltransferase and glucose oxidase. Biotechnol Lett 15:65–70

    Article  CAS  Google Scholar 

  8. Yun JW, Lee MG, Song SK (1994) Batch production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of β-fructofuranosidase and glucose oxidase. J Ferment Bioeng 77:159–163

    Article  CAS  Google Scholar 

  9. Sheu DC, Lio PJ, Chen ST, Lin CT, Duan KJ (2001) Production of fructooligosaccharides in high yield using a mixed enzyme system of β-fructofuranosidase and glucose oxidase. Biotechnol Lett 23:1499–1503

    Article  CAS  Google Scholar 

  10. Sheu DC, Duan KJ, Cheng CY, Bi JL, Chen JY (2002) Continuous production of high-content fructooligosaccharides by a complex cell system. Biotechnol Prog 18:1283–1286

    Article  Google Scholar 

  11. Yang YL, Wang JH, Teng D, Zhang F (2008) Preparation of high-purity fructo-oligosaccharides by Aspergillus japonicus β-fructofuranosidase and successive cultivation with yeast. J Agric Food Chem 56:2805–2809

    Article  CAS  Google Scholar 

  12. Sheu DC, Chang JY, Chen YJ, Lee CW (2013) Production of high-purity neofructooligosaccharides by culture of Xanthophyllomyces dendrorhous. Biores Technol 132:432–435

    Google Scholar 

  13. Linde D, Rodríguez-Colinas B, Estévez M, Poveda A, Plou FJ, Fernández-Lobato M (2012) Analysis of neofructooligosaccharides production mediated by the extracellular β-fructofuranosidase from Xanthophyllomyces dendrorhous. Biores Technol 109:123–130

    Article  CAS  Google Scholar 

  14. Duan KJ, Sheu DC, Chen JS (1993) Purification and characterization of β- fructofuranosidase from Aspergillus japonicus TIT-KJ1. Biosci Biotech Biochem 57:1811–1815

    Article  CAS  Google Scholar 

  15. Holcroft DM, Kader AA (1999) Controlled atmosphere-induced changes in pH and organic acid metabolism may affect color of stored strawberry fruit. Postharvest Biol Tec 17:19–32

    Article  CAS  Google Scholar 

  16. Krajewski V, Simic P, Mouncey NJ, Bringer S, Sahm H, Bott M (2010) Metabolic engineering of Gluconobacter oxydans for improved growth rate and growth yield on glucose by elimination of gluconate formation. Appl Environ Microbiol 76:4369–4376 109:123–130

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Science Council (NSC), Taiwan, under Contract NSC 99-2221-E-036-019.

Author information

Authors and Affiliations

  1. Department of Bioengineering, Tatung University, Taipei, 104, Taiwan

    Dey Chyi Sheu, Jan Yi Chang, Chung Yih Wang & Chang Ta Wu

  2. Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan

    Chen Ji Huang

Authors
  1. Dey Chyi Sheu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jan Yi Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chung Yih Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chang Ta Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chen Ji Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dey Chyi Sheu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sheu, D.C., Chang, J.Y., Wang, C.Y. et al. Continuous production of high-purity fructooligosaccharides and ethanol by immobilized Aspergillus japonicus and Pichia heimii . Bioprocess Biosyst Eng 36, 1745–1751 (2013). https://doi.org/10.1007/s00449-013-0949-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-013-0949-8

Keywords

Search

Navigation