Bioprocess and Biosystems Engineering

, Volume 36, Issue 11, pp 1621–1630 | Cite as

Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis

  • Hyo-Jeong Kim
  • Kwon-Young Choi
  • Da-Hye Jung
  • Joon-Young Jung
  • EunOk Jung
  • Yung-Hun Yang
  • Byung-Gee Kim
  • Min-Kyu Oh
Original Paper

Abstract

Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis.

Keywords

Streptomyces avermitilis Transcriptomics Daidzein Biotransformation 

Supplementary material

449_2013_935_MOESM1_ESM.doc (556 kb)
Supplementary material 1 (DOC 556 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Hyo-Jeong Kim
    • 1
  • Kwon-Young Choi
    • 2
  • Da-Hye Jung
    • 2
  • Joon-Young Jung
    • 1
  • EunOk Jung
    • 2
  • Yung-Hun Yang
    • 3
  • Byung-Gee Kim
    • 2
  • Min-Kyu Oh
    • 1
  1. 1.Department of Chemical and Biological EngineeringKorea UniversitySeoulSouth Korea
  2. 2.School of Chemical and Biological Engineering, Institute of BioengineeringSeoul National UniversitySeoulSouth Korea
  3. 3.Department of Microbial Engineering, College of EngineeringKonkuk UniversitySeoulSouth Korea

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