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Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis

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Abstract

Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis.

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Acknowledgments

The authors wish to acknowledge the financial support of the Seoul R&BD Program (KU080657) and WCU (World Class University) program through the National Research Foundation of Korea (NRF) grant funded by MEST (R322012000102130) and also through Institute of Bioengineering, Seoul National University, Seoul, Korea.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul, 136-713, South Korea

    Hyo-Jeong Kim, Joon-Young Jung & Min-Kyu Oh

  2. School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea

    Kwon-Young Choi, Da-Hye Jung, EunOk Jung & Byung-Gee Kim

  3. Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, South Korea

    Yung-Hun Yang

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  1. Hyo-Jeong Kim
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Correspondence to Min-Kyu Oh.

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H.-J. Kim and K.-Y. Choi contributed equally.

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Kim, HJ., Choi, KY., Jung, DH. et al. Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis . Bioprocess Biosyst Eng 36, 1621–1630 (2013). https://doi.org/10.1007/s00449-013-0935-1

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