Abstract
Enzymatic synthesis of biodiesel by the transesterification of canola oil and methanol in high pressure carbon dioxide [HPCO2: near-critical and supercritical carbon dioxide (NcCO2 and ScCO2)] was optimized using response surface methodology (RSM). RSM based on 5-level-5-factor central composite rotatable design (CCRD) was used to evaluate the effects of temperature, pressure, enzyme loading, substrate molar ratio, and time on the conversion to biodiesel by transesterification. Finally, batch reactions for biodiesel synthesis were preformed in a 100 mL and 7 L high-pressure stirred batch reactors.
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Chen JW, Wu WT (2003) Regeneration of immobilized Candida antarctica lipase for transesterification. J Biosci Bioeng 95(5):466–469
Lee M, Lee J, Lee D, Cho J, Kim S, Park C (2011) Improvement of enzymatic biodiesel production by controlled substrate feeding using silica gel in solvent free system. Enzyme Microb Technol 49(4):402–406
Fukuda H, Kondo A, Noda H (2001) Biodiesel fuel production by transesterification of oils. J Biosci Bioeng 92(5):405–416
Go A, Ko JW, Lee SJ, Kim SW, Han SO, Lee J, Woo HM, Um Y, Nam J, Park C (2012) Process design and evaluation of value-added chemicals production from biomass. Biotechnol Bioproc Eng 17(5):1055–1061
Seong PJ, Jeon BW, Lee M, Cho DH, Kim DK, Jung KS, Kim SW, Han SO, Kim YH, Park C (2011) Enzymatic coproduction of biodiesel and glycerol carbonate from soybean oil and dimethyl carbonate. Enzyme Microb Technol 48(6–7):505–509
Nelson LA, Foglia TA, Marmer WN (1996) Lipase-catalyzed production of biodiesel. J Am Oil Chem Soc 73(9):1191–1195
Shimada Y, Watanabe Y, Sugihara A, Tominaga Y (2002) Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing. J Mol Catal B-Enzym 17(3–6):133–142
Köse Ö, Tuter M, Aksov HA (2002) Immobilized Candida antarctica lipase catalyzed alcoholysis of cotton seed oil in a solvent-free medium. Bioresour Technol 83(2):125–129
Lee JH, Kwon CH, Kwon JW, Park C, Tae B, Kim SW (2009) Biodiesel production from various oils under supercritical fluid condition by Candida antarctica Lipase B using a stepwise reaction method. Appl Biochem Biotech 156(1–3):24–34
Lee M, Park C, Cho J, Lee J, Lee D, Kim S (2010) Enzymatic biodiesel synthesis from canola oil in liquid carbon dioxide. KSBB J 25(4):337–343
Lee M, Lee D, Cho JK, Cho J, Han J, Park C, Kim S (2012) Improved high-pressure enzymatic biodiesel batch synthesis in near-critical carbon dioxide. Bioprocess Biosyst Eng 35(1–2):105–113
Aucoin MG, Legge RL (2001) Effects of supercritical CO2 exposure and depressurization on immobilized lipase activity. Biotechnol Lett 23(22):1863–1870
Lozano P, Avellaneda A, Pascual R, Iborra JL (1996) Stability of immobilized α-chymotrypsin in supercritical carbon dioxide. Biotechnol Lett 18(11):1345–1350
Overmeyer A, Schrader-Lippelt S, Kasche V, Brunner G (1999) Lipase-catalysed kinetic resolution of racemates at temperatures from 40 to 160 °C in supercritical CO2. Biotechnol Lett 21(1):65–69
Nagesha GK, Manohar B, Udaya Sanker K (2004) Enzymatic esterification of free fatty acids of hydrolyzed soy deodorizer distillate in supercritical carbon dioxide. J Supercrit Fluid 32(1–3):137–145
Myers RH (1999) Response surface methodology-current status and future directions. J Qual Technol 31:30–44
Cochran WG, Cox GM (1992) Experimental designs. Wiley, New York
Lee MT, Chen WC, Chou CC (1999) Optimization and kinetic analysis of cholesterol oxidase production by Rhodococcus equi No. 23 in submerged cultures. Enzyme Microb Tech 25(7):598–604
Lui X, He H, Wang Y, Zhu S, Piao X (2007) Transesterification of soybean oil to biodiesel using CaO as a solid base catalyst. Fuel 87(2):216–221
Wang Y, Wu H, Zong MH (2008) Improvement of biodiesel production by lipozyme TLIM-catalyzed methanolysis using response surface methodology and acyl migration enhancer. Bioresour Technol 99(15):7232–7237
Shimada Y, Watanabe Y, Samukawa T, Sugihara A, Noda H, Fukuda H, Tominaga Y (1999) Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase. J Am Oil Chem Soc 76(7):789–793
Noureddini H, Harmeier SE (1998) Enzymatic glycerolysis of soybean oil. J Am Oil Chem Soc 75(10):1359–1365
Acknowledgments
This work was funded by the Industrial Strategic Technology Development Program of the Ministry of Knowledge Economy (MKE), Korea and by the Basic Research Program of the Korea Institute of Industrial Technology (KITECH). This research was also supported by the Knowledge-based Environmental Service Human Resource Development Project of Korea Ministry of Environment (MOE) and the Research Grant from Kwangwoon University in 2013. The 7 L high-pressure stirred batch reactor was provided by Hanil Corporation, Korea.
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Lee, M., Lee, D., Cho, J. et al. Optimization of enzymatic biodiesel synthesis using RSM in high pressure carbon dioxide and its scale up. Bioprocess Biosyst Eng 36, 775–780 (2013). https://doi.org/10.1007/s00449-013-0903-9
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DOI: https://doi.org/10.1007/s00449-013-0903-9