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Immobilization and characterisation of a lipase from a new source, Bacillus sp. ITP-001

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Abstract

A new source of lipase from Bacillus sp. ITP-001 was immobilized by physical adsorption on the polymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) in aqueous solution. The support and immobilized lipase were characterised, compared to the lyophilised lipase, with regard to the specific surface area, adsorption–desorption isotherms, pore volume (Vp) and size (dp) by nitrogen adsorption, differential scanning calorimetry, thermogravimetric analysis, chemical composition analysis, Fourier transform infrared spectroscopy and biochemical properties. The immobilized enzyme displayed a shift in optimum pH towards the acidic side with an optimum at pH 4.0, whereas the optimum pH for the free enzyme was at pH 7.0; the optimum temperature of activity was 80 and 37 °C for the free and immobilized enzyme, respectively. The inactivation rate constant for the immobilized enzyme at 37 °C was 0.0038 h−1 and the half-life was 182.41 h. The kinetic parameters obtained for the immobilized enzyme gave a Michaelis–Menten constant (K m) of 49.10 mM and a maximum reaction velocity (V max) of 205.03 U/g. Furthermore, the reuse of the lipase immobilized by adsorption allowed us to observe that it could be reused for 10 successive cycles, duration of each cycle (1 h), maintaining 33 % of the initial activity.

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Acknowledgments

The authors acknowledge financial assistance from the Brazilian research funding agencies CAPES, CNPq and FAPITEC/SE.

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Correspondence to Cleide M. F. Soares.

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Cabrera-Padilla, R.Y., Albuquerque, M., Figueiredo, R.T. et al. Immobilization and characterisation of a lipase from a new source, Bacillus sp. ITP-001. Bioprocess Biosyst Eng 36, 1385–1394 (2013). https://doi.org/10.1007/s00449-012-0875-1

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  • DOI: https://doi.org/10.1007/s00449-012-0875-1

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