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Production of laccase from Trametes versicolor by solid-state fermentation using olive leaves as a phenolic substrate

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Abstract

The aim of the present study was to investigate whether olive leaves were feasible as a substrate for laccase production by the white-rot fungus Trametes versicolor FPRL 28A INI under solid-state fermentation conditions. Different experiments were conducted to select the variables that allow obtaining high levels of laccase activity. In particular, the effects of the initial moisture content, substrate particle size, supplementation with inorganic and organic nitrogen sources were evaluated. Highest laccase activity (276.62 ± 25.67 U/g dry substrate) was achieved with 80 % initial moisture content and 1.4–1.6 mm particle size of the substrate supplemented with yeast extract (1 % (w/w) nitrogen). Such a high activity was obtained without any addition of inducers.

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Acknowledgments

This work was supported by Ege University Research Fund (İzmir, Turkey, Project no: 009 MUH 008). The authors wish to thank Dr. Tajalli Keshavarz and Christine S. Evans (Westminster University, England) for kindly supplying the fungal strain.

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  1. Institute of Natural and Applied Sciences, Ege University, 35100, Bornova Izmir, Turkey

    Tuğba Aydınoğlu

  2. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova Izmir, Turkey

    Sayıt Sargın

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  1. Tuğba Aydınoğlu
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Correspondence to Sayıt Sargın.

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Aydınoğlu, T., Sargın, S. Production of laccase from Trametes versicolor by solid-state fermentation using olive leaves as a phenolic substrate. Bioprocess Biosyst Eng 36, 215–222 (2013). https://doi.org/10.1007/s00449-012-0777-2

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  • DOI: https://doi.org/10.1007/s00449-012-0777-2

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