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Simultaneous saccharification and fermentation (SSF) of Jatropha curcas shells: utilization of co-products from the biodiesel production process

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Abstract

Jatropha curcas has great potential as an oil crop for use in biodiesel applications, and the outer shell is rich in lignocellulose that may be converted to ethanol, giving rise to the concept of a biorefinery. In this study, two dilute pretreatments of 0.5% H2SO4 and 1.0% NaOH were performed on Jatropha shells with subsequent simultaneous saccharification and fermentation (SSF) of the pretreated water-insoluble solids (WIS) to evaluate the effect of inhibitors in the pretreatment slurry. A cellulase loading of 15 FPU/g WIS, complimented with an excess of cellobiase (19.25 U/g), was used for SSF of either the washed WIS or the original slurry to determine the effect of inhibitors. Ethanol and glucose were monitored during SSF of 20 g of pretreated biomass. The unwashed slurry showed to have a positive effect on SSF efficiency for the NaOH-pretreated biomass. Maximum efficiencies of glucan conversion to ethanol in the WIS were 40.43% and 41.03% for the H2SO4- and NaOH-pretreated biomasses, respectively.

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References

  1. Sims REH, Hastings A, Schlamadinger B, Taylor G, Smith P (2006) Energy crops: current status and future prospects. Glob Change Biol 12:2054–2076

    Article  Google Scholar 

  2. Agencia Nacional de Petroleo (2011) Authorized capacity of biodiesel production plants. http://www.anp.gov.br/?pg=29048&m=&t1=&t2=&t3=&t4=&ar=&ps=&cachebust=1322657707167. Accessed May 2011

  3. Brazilian Federal Government, Ministry of Mines and Energy (2010) Brazilian Energy Balance. Empresa de Pesquisa Energetica, Brazil

  4. Kim S, Dale BE (2004) Global potential bioethanol production from wasted crops and crop residues. Biomass Bioenergy 26:361–375

    Article  Google Scholar 

  5. Öhgren K, Bura R, Lesnicki G, Saddler J, Zacchi G (2007) A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover. Process Biochem 42:834–839

    Article  Google Scholar 

  6. Saha BC, Iten LB, Cotta MA, Wu YV (2005) Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Process Biochem 40:3693–3700

    Article  CAS  Google Scholar 

  7. Karimi K, Emtiazi G, Taherzadeh MJ (2006) Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme Microb Technol 40:138–144

    Article  CAS  Google Scholar 

  8. Saha BC, Cotta MA (2008) Lime pretreatment, enzymatic saccharification and fermentation of rice hulls to ethanol. Biomass Bioenergy 32:971–977

    Article  CAS  Google Scholar 

  9. Linde M, Galbe M, Zacchi G (2007) Simultaneous saccharification and fermentation of steam-pretreated barley straw at low enzyme loadings and low yeast concentration. Enzyme Microb Technol 40:1100–1107

    Article  CAS  Google Scholar 

  10. Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne J (2007) A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol 98:3000–3011

    Article  CAS  Google Scholar 

  11. Adsul MG, Ghule JE, Shaikh H, Singh R, Bastawde KB, Gokhale DV, Varma AJ (2005) Enzymatic hydrolysis of delignified bagasse polysaccharides. Carbohydr Polym 62:6–10

    Article  CAS  Google Scholar 

  12. Abou Kheira AA, Atta NMM (2008) Response of Jatropha curcas L. to water deficit: yield, water use efficiency and oilseed characteristics. Biomass Bioenergy 33:1343–1350

    Article  Google Scholar 

  13. Eggeman T, Elander RT (2005) Process and economic analysis of pretreatment technologies. Bioresour Technol 96:2019–2025

    Article  CAS  Google Scholar 

  14. Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 96:673–686

    Article  CAS  Google Scholar 

  15. Chandel AK, Kapoor RK, Singh A, Kuhad RC (2007) Detoxification of sugarcane bagasse hydrolysate improves ethanol production by Candida shehatae NCIM 3501. Bioresour Technol 98:1947–1950

    Article  CAS  Google Scholar 

  16. Tengborg C, Galbe M, Zacchi G (2001) Reduced inhibition of enzymatic hydrolysis of steam-pretreated softwood. Enzyme Microb Technol 28:835–844

    Article  CAS  Google Scholar 

  17. Öhgren K, Vehmaanperä J, Siika-Aho M, Galbe M, Viikari L, Zacchi G (2007) High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol production. Enzyme Microb Technol 40:607–613

    Article  Google Scholar 

  18. Oberoi HS, Vadlani PV, Brijwani K, Bhargav VK, Patil RT (2010) Enhanced ethanol production via fermentation of rice straw with hydrolysate-adapted Candida tropicalis ATCC 13803. Process Biochem 45:1299–1306

    Article  CAS  Google Scholar 

  19. Vicente MdA, Fietto LG, Castro IdM, Santos ANGd, Coutrim MX, Brandão RL (2006) Isolation of Saccharomyces cerevisiae strains producing higher levels of flavoring compounds for production of “cachaça” the Brazilian sugarcane spirit. Int J Food Microbiol 108:51–59

    Article  CAS  Google Scholar 

  20. US Department of Energy (2006) Theoretical ethanol yield calculator. http://www1.eere.energy.gov/biomass/ethanol_yield_calculator.html. Accessed May 2011

  21. Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268

    Article  CAS  Google Scholar 

  22. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  23. Wallis AFA, Wearne RH, Wright PJ (1996) Chemical analysis of polysaccharides in plantation eucalypt woods and pulps. Appita J 49:258–262

    CAS  Google Scholar 

  24. Horwitz W, Senzel A, Park DL, Reynolds H (eds) (1975) Official methods of analysis of the Association of Official Analytical Chemists, 12th edn. Association of Official Analytical Chemists, Washington

  25. Goldschmid O (1971) Ultraviolet spectra. In: Sarkaned KV, Ludwig CH (eds) Lignins: occurrence, formation, structure and reactions. Wiley, New York

    Google Scholar 

  26. Gomide JL, Demuner BJ (1986) Determinação do teor de lignina em material lenhoso: método Klason modificado. O Papel 47:36–38

    Google Scholar 

  27. Openshaw K (2000) A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass Bioenergy 19:1–15

    Article  Google Scholar 

  28. Martínez-Herrera J, Siddhuraju P, Francis G, Dávila-Ortíz G, Becker K (2006) Chemical composition, toxic/antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico. Food Chem 96:80–89

    Article  Google Scholar 

  29. Fundaçao Centro Tecnológico de Minas Gerais (CETEC) (1983) Programa Energia—Produção de combustíveis líquidos a partir de óleos vegetais. p 152

  30. CETEC (2006) Composição Química da Torta de Pinhão-Manso. www.PinhaoManso.com.br

  31. Zhu S, Wu Y, Yu Z, Chen Q, Wu G, Yu F, Wang C, Jin S (2006) Microwave-assisted alkali pre-treatment of wheat straw and its enzymatic hydrolysis. Biosyst Eng 94:437–442

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the Brazilian foundations CNPq and CAPES for their financial support.

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Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, 36570-000, Brazil

    Evan Michael Visser & Valéria Monteze Guimarães

  2. Departamento de Engenharia Agrícola, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, 36570-000, Brazil

    Delly Oliveira Filho & Marcio Arêdes Martins

  3. Departamento de Microbiologia, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, 36570-000, Brazil

    Marcos Rogério Tótola

Authors
  1. Evan Michael Visser
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  2. Delly Oliveira Filho
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  3. Marcos Rogério Tótola
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  4. Marcio Arêdes Martins
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  5. Valéria Monteze Guimarães
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Correspondence to Valéria Monteze Guimarães.

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Visser, E.M., Filho, D.O., Tótola, M.R. et al. Simultaneous saccharification and fermentation (SSF) of Jatropha curcas shells: utilization of co-products from the biodiesel production process. Bioprocess Biosyst Eng 35, 801–807 (2012). https://doi.org/10.1007/s00449-011-0662-4

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  • DOI: https://doi.org/10.1007/s00449-011-0662-4

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