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Development of a protocol for supercritical carbon dioxide extraction of ubiquinone-10 from dried biomass of Pseudomonas diminuta

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Abstract

Ubiquinone (Coenzyme Q10, CoQ10), a yellow-to-orange-colored lipophilic substance having nutraceutical value, was extracted from dried biomass of Pseudomonas diminuta using supercritical carbon dioxide (SC-CO2). The effect of different operational parameters (temperature, pressure, and extraction time) and addition of co-solvent on SC-CO2 extraction of CoQ10 was studied in detail. The solubility parameter of CoQ10, CO2, and CO2 with ethanol and methanol as co-solvents was calculated and validated with experimental results. Theoretically, ethanol and methanol had significant effect as co-solvent, and the difference between the two was only marginal. A maximum recovery of 22.33% was obtained using pure SC-CO2 at 40 °C, 150 bar, and run time of 60 min. Ethanol as co-solvent at 3 mL/g of dried biomass at 40 °C and 150 bar increased the recovery from 22.33 to 68.57%. Further optimization of the extraction conditions by Box–Behnken design effectively increased the recovery to 96.2%. The optimized conditions were a temperature of 38 °C, pressure of 215 bar, and run time of 58 min.

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Acknowledgments

Authors are grateful to the Council of Scientific and Industrial Research (CSIR), India, for providing financial assistance during the course of this research.

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Authors and Affiliations

  1. Food Engineering and Technology Department, Institute of Chemical Technology, Mumbai, 400 019, India

    Mahesh V. Bule & Rekha S. Singhal

  2. Bioprocessing and Bioproduct Engineering Laboratory, Biological System Engineering Department, Washington State University, Pullman, WA, 99164, USA

    Mahesh V. Bule

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  1. Mahesh V. Bule
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  2. Rekha S. Singhal
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Correspondence to Mahesh V. Bule.

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Bule, M.V., Singhal, R.S. Development of a protocol for supercritical carbon dioxide extraction of ubiquinone-10 from dried biomass of Pseudomonas diminuta . Bioprocess Biosyst Eng 35, 809–816 (2012). https://doi.org/10.1007/s00449-011-0661-5

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