Ethanol was produced using the simultaneous saccharification and fermentation (SSF) method with macroalgae polysaccharide from the seaweed Saccharina japonica (Sea tangle, Dasima) as biomass. The seaweed was dried by hot air, ground with a hammer mill and filtered with a 200-mesh sieve prior to pretreatment. Saccharification was carried out by thermal acid hydrolysis with H2SO4 and the industrial enzyme, Termamyl 120 L. To increase the yield of saccharification, isolated marine bacteria were used; the optimal saccharification conditions were 10% (w/v) seaweed slurry, 40 mM H2SO4 and 1 g dcw/L isolated Bacillus sp. JS-1. Using this saccharification procedure, the reducing sugar concentration and viscosity were 45.6 ± 5.0 g/L and 24.9 cp, respectively, and the total yield of the saccharification with optimal conditions and S. japonica was 69.1%. Simultaneous saccharification and fermentation was carried out for ethanol production. The highest ethanol concentration, 7.7 g/L (9.8 ml/L) with a theoretical yield of 33.3%, was obtained by SSF with 0.39 g dcw/L Bacillus sp. JS-1 and 0.45 g dcw/L of the yeast, Pichia angophorae KCTC 17574.
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This research was supported by a grant from the Development of Marine-Bioenergy Program Funded by the Ministry of Land, Transport and Maritime Affairs of the Korean Government. Ji-Suk Jang and YuKyeong Cho were financially supported by the Brain Busan 21 HRD Project of the Metropolitan City of Busan.
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Jang, JS., Cho, Y., Jeong, GT. et al. Optimization of saccharification and ethanol production by simultaneous saccharification and fermentation (SSF) from seaweed, Saccharina japonica . Bioprocess Biosyst Eng 35, 11–18 (2012). https://doi.org/10.1007/s00449-011-0611-2
- Saccharina japonica
- Thermal acid hydrolysis
- Pichia angophorae