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Optimization of pretreatment and saccharification for the production of bioethanol from water hyacinth by Saccharomyces cerevisiae

Bioprocess and Biosystems Engineering volume 35, pages 35–41 (2012)Cite this article

Abstract

Alkaline–oxidative (A/O) pretreatment and enzymatic saccharification were optimized for bioethanol fermentation from water hyacinth by Saccharomyces cerevisiae. Water hyacinth was subjected to A/O pretreatment at various NaOH and H2O2 concentrations and reaction temperatures for the optimization of bioethanol fermentation by S. cerevisiae. The most effective condition for A/O pretreatment was 7% (w/v) NaOH at 100 °C and 2% (w/v) H2O2. The carbohydrate content was analyzed after reaction at various enzyme concentrations and enzyme ratios using Celluclast 1.5 L and Viscozyme L to determine the effective conditions for enzymatic saccharification. After ethanol fermentation using S. cerevisiae KCTC 7928, the concentration of glucose, ethanol and glycerol was analyzed by HPLC using a RI detector. The yield of ethanol in batch fermentation was 0.35 g ethanol/g biomass. Continuous fermentation was carried out at a dilution rate of 0.11 (per h) and the ethanol productivity was 0.77 [g/(l h)].

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Acknowledgments

This research was supported by Inha University.

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  1. Department of Biological Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, 402-751, Korea

    Deuk Joo Ahn, Se Kyung Kim & Hyun Shik Yun

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  1. Deuk Joo Ahn
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  2. Se Kyung Kim
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  3. Hyun Shik Yun
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Correspondence to Hyun Shik Yun.

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Ahn, D.J., Kim, S.K. & Yun, H.S. Optimization of pretreatment and saccharification for the production of bioethanol from water hyacinth by Saccharomyces cerevisiae . Bioprocess Biosyst Eng 35, 35–41 (2012). https://doi.org/10.1007/s00449-011-0600-5

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  • DOI: https://doi.org/10.1007/s00449-011-0600-5

Keywords

  • Bioethanol
  • Saccharomyces cerevisiae
  • Water hyacinth
  • Eichhornia crassipes
  • Fermentation