Bioprocess and Biosystems Engineering

, Volume 35, Issue 3, pp 433–440 | Cite as

Column bioleaching of low-grade mining ore containing high level of smithsonite, talc, sphaerocobaltite and azurite

  • Sadia Ilyas
  • Ruan ChiEmail author
  • H. N. Bhatti
  • I. A. Bhatti
  • M. A. Ghauri
Original Paper


Present work describes the bioleaching potential of metals from low-grade mining ore containing smithsonite, sphaerocobaltite, azurite and talc as main gangue minerals with adapted consortium of Sulfobacillus thermosulfidooxidans strain-RDB and Thermoplasma acidophilum. Bioleaching potential improved markedly by added energy source, acid preleaching and adaptation of microbial consortium with mixed metal ions. During whole leaching period including acid preleaching stage of 960 h and bioleaching stage of 212 days about 76% Co, 70% Zn, 84% Cu, 72% Ni and 63% Fe leached out.


Column bioleaching Low-grade sulfide ore Consortium of moderate thermophiles 



This work was supported by Changjiang scholars and innovation research team in universities (IRT0974), Higher Education Commission of Pakistan and National Institute for Biotechnology and Genetic Engineering (Industrial biotechnology division) of Pakistan.

Supplementary material

449_2011_582_MOESM1_ESM.doc (512 kb)
Supplementary material 1 (DOC 509 kb)
449_2011_582_MOESM2_ESM.tif (4.2 mb)
Supplementary material 2 (TIFF 4268 kb)
449_2011_582_MOESM3_ESM.jpg (72 kb)
Supplementary material 3 (JPG 72 kb)


  1. 1.
    Bosecker K (1985) Leaching of lateritic nickel ores with heterotrophic microorganisms, Proceedings of the sixth international symposium on biohydrometallurgy. Fund. Appl. Biohydrometal. Vancouver, B.C., Canada, pp 367–382Google Scholar
  2. 2.
    Tzeferis PG, Agatzinin LS (1994) Leaching of nickel and iron from Greek non-sulphide nickeliferous ores by organic acids. Hydrometallurgy 36:345–360CrossRefGoogle Scholar
  3. 3.
    Valix M, Usai F, Malik V (2000) Fungal bioleaching of low grade laterite ores. Miner Eng 14:197–203CrossRefGoogle Scholar
  4. 4.
    Valix M, Usai F, Malik V (2000) The electra-sorption properties of nickel on laterite gangue leached with an organic chelating acid. Miner Eng 14:205–215CrossRefGoogle Scholar
  5. 5.
    Ilyas S, Anwar MA, Niazi SB, Ghauri MA, Khalid AM (2007) Microbial leaching of iron from pyrite by moderate thermophile chemolithotrophic bacteria. J Res Sci 18:159–166Google Scholar
  6. 6.
    Devasia P, Natarajan KA (2004) Bacterial leaching: biotechnology in mining industry. Resonance 27–34Google Scholar
  7. 7.
    Pradhan N, Das B, Gahan CS, Kar RN, Sukla LB (2006) Beneficiation of iron ore slime using Aspergillus niger and Bacillus circulans. Bioresour Technol 97:1876–1879CrossRefGoogle Scholar
  8. 8.
    Rawlings DE (2004) Microbially assisted dissolution of minerals and its use in the mining industry. Pure Appl Chem 76:847–859CrossRefGoogle Scholar
  9. 9., bulletin, China mining. 28 September (2006)
  10. 10.
    Rossi G (1990) Biohydrometallurgy. McGraw-Hill Book Company, New YorkGoogle Scholar
  11. 11.
    Olson GJ, Brierley JA, Brierley CL (2003) Bioleaching review part B: progress in bioleaching: applications of microbial processes by the minerals industries. Appl Microbiol Biotechnol 63:249–257CrossRefGoogle Scholar
  12. 12.
    Ilyas S, Anwar MA, Niazi SB, Ghauri MA, Ahmad B, Khan KM (2008) Bioleaching of Pb-Zn ore by moderate thermophilic chemolithotrophic bacteria. J Chem Soc Pak 30:61–68Google Scholar
  13. 13.
    Ilyas S, Bhatti HN, Bhatti IA, Sheikh MA, Ghauri MA (2010) Bioleaching of metal ions from low grade ore: process optimization by using orthogonal experimental array design. Afr J Biotechnol 19:2801–2810Google Scholar
  14. 14.
    Ilyas S, Chi R, Bhatti HN, Ghauri MA, Anwar MA (2010) Column bioleaching of metals from electronic scrap. Hydrometallurgy 101:135–140CrossRefGoogle Scholar
  15. 15.
    Johnson DB, Macvicar JHM, Rolfe S (1987) A new medium for the isolation and enumeration of Thiobacillus ferrooxidans and acidophilic heterotrophic bacteria. J Microbiol Meth 7:9–18CrossRefGoogle Scholar
  16. 16.
    Karamanev DG, Nikolov LN, Mamatarkova V (2002) Rapid simultaneous quantitative determination of ferric and ferrous ions in drainage waters and similar solutions. Miner Eng 15:341–346CrossRefGoogle Scholar
  17. 17.
    Silver S (1996) Bacterial resistance to toxic metal ions, a review. Gene 179:9–19CrossRefGoogle Scholar
  18. 18.
    Nies DH, Silver S (1995) Ion efflux systems involved in bacterial metal resistances. J Ind Microbiol 14:186–199CrossRefGoogle Scholar
  19. 19.
    Olafson RW, Mccubbin WD, Kay CM (1988) Primary and secondary structural analysis of a unique prokaryotic metallothionein from a Synechococcus sp. Cyanobacterium. Biochem J 251:691–699Google Scholar
  20. 20.
    Robinson NJ, Gupta A, Fordham SAP, Croy RRD, Whitton BA, Huckle JW (1990) Prokaryotic metallothionein gene characterization and expression: chromosome crawling by ligation-mediated PCR. Proc R Soc Lond 242:241–247CrossRefGoogle Scholar
  21. 21.
    Zhen S, Yan Z, Zhang Y, Wang J, Campbell M, Qin W (2009) Column bioleaching of a low grade nickel-bearing sulphide ore containing high magnesium as olivine, chlorite and antigorite. Hydrometallurgy 96:337–341CrossRefGoogle Scholar
  22. 22.
    Kelley BC, Touvinen OH (1988) Microbiological oxidation of minerals in mine tailings. In: Solomons W, Foerstner V (eds) Chemistry and biology of solid wastes. Springer, Berlin, pp 33–53Google Scholar
  23. 23.
    Nemati M, Webb C (1997) A kinetic model for biological oxidation of ferrous iron by Thiobacillus ferrooxidans. Biotechnol Bioeng 53:478–486CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Sadia Ilyas
    • 1
  • Ruan Chi
    • 1
    Email author
  • H. N. Bhatti
    • 2
  • I. A. Bhatti
    • 2
  • M. A. Ghauri
    • 3
  1. 1.Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, School of Chemical Engineering and PharmacyWuhan Institute of TechnologyWuhanChina
  2. 2.Department of Chemistry and BiochemistryUniversity of AgricultureFaisalabadPakistan
  3. 3.National Institute for Biotechnology and Genetic Engineering (NIBGE)FaisalabadPakistan

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