Abstract
This paper describes the performance of a novel bio-reactor system, the membrane-integrated fermentation reactor (MFR), for efficient continuous fermentation. The MFR, equipped with an autoclavable polyvinylidene difluoride membrane, has normally been used for biological wastewater treatment. The productivity of the MFR system, applied to the continuous production of pyruvic acid by the yeast Torulopsis glabrata, was remarkably high. The volumetric productivity of pyruvic acid increased up to 4.2 g/l/h, about four times higher than that of batch fermentation. Moreover, the membrane was able to filter fermentation broth for more than 300 h without fouling even though the cell density of the fermentation broth reached 600 as OD660. Transmembrane pressure, used as an indicator of membrane fouling, remained below 5 kPa throughout the continuous fermentation. These results clearly indicate that the MFR system is a simple and highly efficient system that is applicable to the fermentative production of a range of biochemicals.
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References
Chang HN, Yoo IK, Kim BS (1994) High density cell culture by membrane-based cell recycle. Biotechnol Adv 12:467–487
Charcosset C (2006) Membrane processes in biotechnology: an overview. Biotechnol Adv 24:482–492
Stephenson T, Judd S, Jefferson B, Brindle K (2000) Membrane bioreactors for wastewater treatment. IWA Publishing, London
Yamamoto K, Hiasa M, Mahmood T, Matsuno T (1989) Direct solid–liquid separation using hollow fiber membrane in an activated-sludge aeration tank. Water Sci Technol 21:43–54
Meng F, Chae SR, Drews A, Kraume M, Shin HS, Yang F (2009) Recent advances in membrane bioreactors (MBRs): membrane fouling and membrane material. Water Res 43:1489–1512
Yonehara T, Miyata R (1994) Fermentative production of pyruvate from glucose by Torulopsis glabrata. J Ferm Bioeng 78:155–159
Miyata R, Yonehara T (2000) Improvement of fermentative production of pyruvate from glucose by Torulopsis glabrata. J Ferm Bioeng 82:475–479
Henmi M, Fusaoka Y, Matsuka N, Kurihara M (2003) Development of flat sheet immersed membrane module and operation performance in MBR International Desalination Association, World Congress on Desalination and Water Reuse September 28–October 3, 2003, Bahama
Yonehara T, Miyata R, Matsuno H, Goto M, Yahanda S (2000) Development of fermentative production of pyruvate by metabolic control. Seibutsu Kogakkaishi 78:56–62
Clech PL, Chen V, Fane T (2006) Fouling in membrane bioreactors used for wastewater treatment—a review. J Memb Sci 284:17–53
Ognier S, Wisniewski C, Grasmick A (2002) Characterization and modeling of fouling in membrane bioreactor. Desalination 146:141–147
Hua Q, Yang C, Shimizu K (1999) Metabolic flux analysis for efficient pyruvate fermentation using vitamin-autotrophic yeast of Torulopsis grabarata. J Biosci Bioeng 87:206–213
Minegishi S, Matsuka N (2007) Advanced fouling resistant PVDF hollow fiber membrane modules “Torayfil HFM, HFS, HFU”. Membrane 32:311–314
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This work was partially supported by a grant from the New Energy and Industrial Technology Development Organization (NEDO), Japan.
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Sawai, H., Mimitsuka, T., Minegishi, Si. et al. A novel membrane-integrated fermentation reactor system: application to pyruvic acid production in continuous culture by Torulopsis glabrata . Bioprocess Biosyst Eng 34, 721–725 (2011). https://doi.org/10.1007/s00449-011-0521-3
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DOI: https://doi.org/10.1007/s00449-011-0521-3