Bioprocess and Biosystems Engineering

, Volume 34, Issue 4, pp 505–513 | Cite as

Fabrication of porous polycaprolactone/hydroxyapatite (PCL/HA) blend scaffolds using a 3D plotting system for bone tissue engineering

  • Su A ParkEmail author
  • Su Hee Lee
  • Wan Doo Kim
Original Paper


For tissue engineering and regeneration, a porous scaffold with interconnected networks is needed to guide cell attachment and growth/ingrowth in three-dimensional (3D) structure. Using a rapid prototyping (RP) technique, we designed and fabricated 3D plotting system and three types of scaffolds: those from polycaprolactone (PCL), those from PCL and hydroxyapatite (HA), and those from PCL/HA and with a shifted pattern structure (PCL/HA/SP scaffold). Shifted pattern structure was fabricated to increase the cell attachment/adhesion. The PCL/HA/SP scaffold had a lower compressive modulus than PCL and PCL/HA scaffold. However, it has a better cell attachment than the scaffolds without a shifted pattern. MTT assay and alkaline phosphatase activity results for the PCL/HA/SP scaffolds were significantly enhanced compared to the results for the PCL and PCL/HA scaffolds. According to their degree of cell proliferation/differentiation, the scaffolds were in the following order: PCL/HA/SP > PCL/HA > PCL. These 3D scaffolds will be applicable for tissue engineering based on unique plotting system.


Polycaprolactone Hydroxyapatite Scaffold Plotting system 


  1. 1.
    Hutmacher DW, Schantz T, Zein I, Ng KW, Teoh SH, Tan KC (2001) Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. J Biomed Mater Res A 55:203–216CrossRefGoogle Scholar
  2. 2.
    Woodfield TBF, Malda J, De Wijn J, Péters F, Riesle J, Van Blitterswijk CA (2004) Design of porous scaffolds for cartilage tissue engineering using a three-dimensional fiber-deposition technique. Biomaterials 25:4149–4161CrossRefGoogle Scholar
  3. 3.
    Sachlos E, Czernuszka JT (2003) Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. Eur Cells Mater 5:29–40Google Scholar
  4. 4.
    Hollister SJ (2005) Porous scaffold design for tissue engineering. Nat Mater 4:518–524CrossRefGoogle Scholar
  5. 5.
    Williams JM, Adewunmi A, Schek RM, Flanagan CL, Krebsbach PH, Feinberg SE, Hollister SJ, Das S (2005) Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. Biomaterials 26:4817–4827CrossRefGoogle Scholar
  6. 6.
    Mironov V, Boland T, Trusk T, Forgacs G, Markwald RR (2003) Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 22:157–161CrossRefGoogle Scholar
  7. 7.
    Seitz H, Rieder W, Irsen S, Leukers B, Tille C (2005) Three-dimensional printing of porous ceramic scaffolds for bone tissue engineering. J Biomed Mater Res B 74b:782–788CrossRefGoogle Scholar
  8. 8.
    Hutmacher DW, Sittinger M, Risbud MV (2004) Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends Biotechnol 22:354–362CrossRefGoogle Scholar
  9. 9.
    Khalil S, Nam J, Sun W (2005) Multi-nozzle deposition for construction of 3D biopolymer tissue scaffolds. Rapid Prototyping J 11:9–17CrossRefGoogle Scholar
  10. 10.
    Sun W, Darling A, Starly B, Nam J (2004) Computer-aided tissue engineering: overview, scope and challenges. Biotechnol Appl Biochem 39:29–47CrossRefGoogle Scholar
  11. 11.
    Sun W, Lal P (2002) Recent development on computer aided tissue engineering: a review. Comput Methods Programs Biomed 67:85–103CrossRefGoogle Scholar
  12. 12.
    Hutmacher DW (2000) Scaffolds in tissue engineering bone and cartilage. Biomaterials 21:2529–2543CrossRefGoogle Scholar
  13. 13.
    Pfister A, Landers R, Laib A, Hübner U, Schmelzeisen R, Mülhaupt R (2004) Biofunctional rapid prototyping for tissue-1 engineering applications: 3D bioplotting versus 3D printing. J Polym Sci Pol Chem 42:624–638CrossRefGoogle Scholar
  14. 14.
    Lee M, Dunn JCY, Wu BM (2005) Scaffold fabrication by indirect three dimensional printing. Biomaterials 26:4281–4289CrossRefGoogle Scholar
  15. 15.
    Vozzi G, Flaim C, Ahluwalia A, Bhatia S (2003) Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition. Biomaterials 24:2533–2540CrossRefGoogle Scholar
  16. 16.
    Zein I, Hutmacher DW, Tan KC, Teoh SH (2002) Fused deposition modeling of novel scaffold architectures for tissue engineering applications. Biomaterials 23:1169–1185CrossRefGoogle Scholar
  17. 17.
    Landers R, Hübner U, Schmelzeisen R, Mülhaupt R (2002) Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. Biomaterials 23:4437–4447CrossRefGoogle Scholar
  18. 18.
    Hoque ME, Hutmacher DW, Feng W, Li S, Huang MH, Vert M, Wong YS (2005) Fabrication using a rapid prototyping system and in vitro characterization of PEG–PCL–PLA scaffolds for tissue engineering. J Biomater Sci 16:1595–1610CrossRefGoogle Scholar
  19. 19.
    Wang X, Yan Y, Zhang R (2007) Rapid prototyping as a tool for manufacturing bioartificial livers. Trends Biotechnol 25:505–513CrossRefGoogle Scholar
  20. 20.
    Schantz JT, Brandwood A, Hutmacher DW, Khor HL, Bittner K (2005) Osteogenic differentiation of mesenchymal progenitor cells in computer designed fibrin–polymer–ceramic scaffolds manufactured by fused deposition modeling. J Mater Sci 16:807–819CrossRefGoogle Scholar
  21. 21.
    Partee B, Hollister SJ, Das S (2006) Selective laser sintering process optimization for layered manufacturing of CAPA® 6510 polycaprolactone bone tissue engineering scaffolds. J Manuf Sci Eng 128:531–540CrossRefGoogle Scholar
  22. 22.
    Lee JH, Park SA, Park KE, Kim JH, Kim KS, Lee JH, Kim WD (2010) Fabrication and characterization of 3D scaffold using 3D plotting system. Chin Sci Bull 55:94–98CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Nature-Inspired Mechanical System Team, Nano Convergence and Manufacturing Systems Research DivisionKorea Institute of Machinery and MaterialsDaejeonKorea

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