Skip to main content
SpringerLink
Log in

Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

This work aimed to develop the submerged cultivation conditions for improved exopolysaccharides (EPS) production by Armillaria luteo-virens Sacc. The effects of culture temperature, aeration rate, inoculum level, initial pH, and additives on EPS formation and mycelial growth are investigated. The aeration rate, initial pH, and inoculum level significantly affected EPS production under the submerged cultivation. The developed conditions were as follows: cultivation temperature 23 °C, initial pH 5.0, aeration rate 0.5 vvm, 0.5% Tween 80, inoculum level 5% (v/v), and shaking speed 120 r/min. Under the developed conditions, the highest EPS production was 13.01 g/L at 5 days culture time. EPS production was examined in a 5 L bioreactor, and an unstructured kinetic model for EPS formation was well developed. The verified investigations in the large-scale cultivation system showed that the developed models are able to predict the submerged cultivation process of EPS formation. Current results revealed that the submerged cultivation conditions can be utilized to control EPS production, and the unstructured models developed are suitable for explaining EPS production by A. luteo-virens Sacc QH in a large-scale cultivation bioreactor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

α :

Growth-associated product formation coefficient (g/g)

β :

Non-growth-associated product formation coefficient (g/g h)

γ, δ:

Parameters in Luedeking-Piret-like equation for substrate uptake [g S (g cell)−1, g S (g cell)−1 h−1, respectively]

μ :

Specific growth rate (h−1)

μ max :

Maximum specific growth rate (h−1)

References

  1. Lee SY, Kang TS (1996) Production conditions and characterization of the exo-polymer produced by submerged cultivation of Ganoderma lucidum. Korean J Microbiol Biotechnol 24:111–118

    Google Scholar 

  2. Kim DH, Shim SB, Kim NJ, Jang IS (1999) β-Glucuronidase inhibitory activity and hepatoprotective effect of Ganoderma lucidum. Biol Pharm Bull 22:162–164

    CAS  Google Scholar 

  3. Yang BK, Ha JY, Jeong SC, Das S, Yun JW, Lee YS, Choi JW, Song CH (2000) Production of exo-polymers by submerged mycelial culture of Cordyceps militaris and its hypolipidemic effect. J Microbiol Biotechnol 10:784–788

    CAS  Google Scholar 

  4. Yang FC, Ke YF, Kuo SS (2000) Effect of fatty acids on the mycelial growth and polysaccharides formation by Ganoderma lucidum in shake-flask cultures. Enzyme Microbiol Technol 27:295–301

    Article  CAS  Google Scholar 

  5. Song CH, Jeon YJ, Yang BK, Ra KS, Sung JM (1998) The anticomplementary activity of exo-polymers produced from submerged mycelial cultures of higher fungi with particular reference to Cordyceps militaris. J Microbiol Biotechnol 8:536–539

    CAS  Google Scholar 

  6. Kim DH, Yang BK, Jeong SC, Park JB, Cho SP, Das S, Yun JW, Song CH (2001) Production of a hypoglycemic, extracellular polysaccharide from the submerged culture of the mushroom, Phellinus linteus. Biotechnol Lett 23:513–517

    Article  CAS  Google Scholar 

  7. Yu L, Shen YS, Li G, Wu JM, Xie QY (2006) Study on the effects of different culturing methods of intrapolysaccharides synthesis for Armiilaria mellea and its antivertigo function. Edible Fungi China 25:37–39

    CAS  Google Scholar 

  8. Watanate N, Obuchi T, Tamai M, Araki H, Omura S, Yang JS, Yu DQ, Liang XT, Huan JH (1990) A novel N-substituted adenosine isolated from Mi Huan Jun (Armiillaria mellea) a cerebral-protecting compound. Planata Medica 56:48–52

    Article  Google Scholar 

  9. Chen SF, Bi YR, Chen GC (2004) Studies on antioxidative and antitumor activities for two wild edible fungi. Edible Fungi China 24:58–63

    CAS  Google Scholar 

  10. Yang JS, Chen YW, Feng XZ, Yu DQ, He CH, Zheng QT, Yang J, Liang XT (1989) Isolation and structure elucidation of Armillaricin. Planta Med 55:564–565

    Article  CAS  Google Scholar 

  11. Feng K, Liu QH, Ng TB, Liu HZ, Li JQ, Chen G et al (2006) Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens. Biochem Biophys Res Commun 345:1573–1578

    Article  CAS  Google Scholar 

  12. Yang FC, Lian CB (1998) The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged cultures. Process Biochem 33:547–553

    Article  CAS  Google Scholar 

  13. Bae JT, Sinha J, Park JP, Song CH, Yun JW (2000) Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. J Microb Biotechnol 10:482–487

    CAS  Google Scholar 

  14. Jonathan SG, Fasidi IO (2001) Studies on phytohormones, vitamins and mineral element requirements of Lentinus subnudus and Schizophyllum commune from Nigeria. Food Chem 75:303–307

    Article  CAS  Google Scholar 

  15. Jonathan SG, Fasidi IO (2001) Effect of carbon, nitrogen and mineral sources on growth of Psathyerella atroumbonata (Pegler), a Nigerian edible mushroom. Food Chem 72:479–483

    Article  CAS  Google Scholar 

  16. Kim SW, Hwang HJ, Xu CP, Na YS, Song SK, Yun JW (2002) Influence of nutritional conditions on the mycelial growth and exopolysaccharide production in Paecilomyces sinclairii. Lett Appl Microb 34:389–393

    Article  CAS  Google Scholar 

  17. Papagianni M (2004) Fungal morphology and metabolite production in submerged mycelial processes. Biotechnol Adv 22:189–259

    Article  CAS  Google Scholar 

  18. Jiao Y, Chen Q, Zhou J, Zhang H, Chen H (2008) Improvement of exo-polysaccharides production and modeling kinetics by Armilaria luteo-virens Sacc in submerged cultivation. LWT Food Sci Technol 41:1694–1700

    Article  CAS  Google Scholar 

  19. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1945) Calorimetric method for determination of sugar and related substance. Anal Chem 28:350–356

    Article  Google Scholar 

  20. Hsieh C, Liu CJ, Tseng MH, Lo CT, Yang YC (2006) Effect of olive oil on the production of mycelial biomass and polysaccharides of Grifola frondosa under high oxygen concentration aeration. Enzyme Microbiol Technol 39:434–439

    Article  CAS  Google Scholar 

  21. Sinclair CG, Kristiansen B (1987) Fermentation kinetics and modeling. Open University Press, Milton Keynes, p 156

    Google Scholar 

  22. Malchandani A, Luong JHT, Leduy A (1998) Batch kinetics of microbial polysaccharide biosynthesis. Biotechnol Bioeng 32:639–646

    Article  Google Scholar 

  23. Montgomery DC (1997) Response surface methods and other approaches to process optimization. In: Montgomery DC (ed) Design and analysis of experiments. Wiley, New York, pp 427–510

    Google Scholar 

  24. Shih IL, Pan K, Hsieh C (2006) Influence of nutritional components and oxygen supply on the mycelial growth and bioactive metabolites production in submerged culture of Antrodia cinnamomea. Process Biochem 41:1129–1135

    Article  CAS  Google Scholar 

  25. Yang FC, Huang HC, Yang MJ (2003) The influence of environmental conditions on the mycelial growth of Antrodia cinnamomea in submerged cultures. Enzyme Microbiol Technol 33:395–402

    Article  CAS  Google Scholar 

  26. Shu CH, Lung MY (2004) Effect of pH on the production and molecular weight distribution of exopolysaccharide by Antrodia camphorata in batch cultures. Process Biochem 39:931–937

    Article  CAS  Google Scholar 

  27. Wang Y, McNeil B (1995) pH effects on exopolysaccharide and oxalic acid production in cultures of Sclerotium glucanicum. Enzyme Microbiol Technol 17:124–130

    Article  CAS  Google Scholar 

  28. Gerlach SR, Siedenberg D, Gerlach D, Schtigerl K, Giuseppin MLF, Hunik J (1998) Influence of reactor systems on the morphology of Aspergillus awamori. Application of neural network and cluster analysis for characterization of fungal morphology. Process Biochem 33:601–615

    Article  CAS  Google Scholar 

  29. Xu CP, Yun JW (2004) Influence of aeration on the production and the quality of the exopolysaccharides from Paecilomyces tenuipes C240 in a stirred-tank fermenter. Enzyme Microbiol Technol 35:33–39

    Article  Google Scholar 

  30. Xiao JH, Chen DX, Xiao Y et al (2004) Optimization of submerged culture conditions for mycelial polysaccharide production in Cordyceps jiangxiensis. J Process Biochem 39:423–426

    Google Scholar 

  31. Wu JZ, Cheung PCK, Wong K-H, Huang N-L (2003) Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer part D physical and chemical factors affecting the rate of mycelial growth and bioconoersion efficiency. Food Chem 81:389–393

    Article  CAS  Google Scholar 

  32. Roukas T, Mantzouridou F (2001) Effect of the aeration rate on pullulan production and fermentation broth rheological properties in an airlift reactor. J Chem Technol Biotechnol 76(4):371–376

    Article  CAS  Google Scholar 

  33. Gibbs PA, Seviour RJ (1996) Does the agitation rate and/or oxygen saturation influence exopolysaccharide production by Aureobasidium pullulans in batch culture? Appl Microbiol Biotechnol 46:503–510

    Article  CAS  Google Scholar 

  34. Stasinopoulos SJ, Seviour RJ (1990) Stimulation of exopolysaccharide production in the fungus Acremonium persicinum with fatty acids. Biotechnol Bioeng 36:778–782

    Article  CAS  Google Scholar 

  35. Fukushima Y, Itoh H, Fukase T, Motai H (1991) Stimulation of protease production by Aspergillus oryzae with oils in continuous culture. Appl Microbiol Biotechnol 34:586–590

    Article  CAS  Google Scholar 

  36. Chen HB, Huang HC, Chen CI IYP, Liu YC (2010) The use of additives as the stimulator on mycelial biomass and exopolysaccharide productions in submerged culture of Grifola umbellata. Bioprocess Biosyst Eng 33:401–406

    Article  CAS  Google Scholar 

  37. Choi KO, Song SH, Yoo YJ (2004) Permeabilization of Ochrobactrum anthropi SY509 cells with organic solvents for whole cell biocatalyst. Biotechnol Bioprocess Eng 9:147–150

    Article  CAS  Google Scholar 

  38. De Leon A, Garcia B, Barba de la rosa AP, Villasenor F, Estrada A, Lopezrevilla R (2003) Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized with organic solvents. Process Biochem 39:301–305

    Article  Google Scholar 

  39. Mazutti MA, Corazza ML, Filho FM, Rodrigues MI, Corazza FC, Treichel H (2009) Inulinase production in a batch bioreactor using agroindustrial residues as the substrate: experimental data and modeling. Bioprocess Biosyst Eng 32:85–95

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by Zhejiang Scientific Technology Project (2009C32098) and the National Nature Science Foundation of China (No. 20806069).

Author information

Authors and Affiliations

  1. College of Chemistry and Biology Science, Lishui University, Lishui, 323000, China

    De Qin Xu

  2. Department of Food Science and Nutrition, Zhejiang University, Kaixuan Rd. 268, Hangzhou, 310029, People’s Republic of China

    Ming Liang Fu, Qi He Chen & Jing Liu

Authors
  1. De Qin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ming Liang Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qi He Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qi He Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, D.Q., Fu, M.L., Chen, Q.H. et al. Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling. Bioprocess Biosyst Eng 34, 103–111 (2011). https://doi.org/10.1007/s00449-010-0451-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-010-0451-5

Keywords

Search

Navigation