Abstract
The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH4 reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris–HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thrombin cluster that resulted in the resonance scattering intensity (I 560 nm) increasing at 560 nm. The increased intensity ΔI 560 nm was linear to the thrombin concentration in the range of 0.115–6.93 nmol/L, with a regression equation of ΔI 560 nm = 53.0 C + 2.5, a correlation coefficient of 0.9989, and a detection limit of 13 pmol/L. This method was applied to detect thrombin in human plasma samples, with satisfactory results.
Similar content being viewed by others
References
Brummel ZK, Undas A, Orfeo T, Gissel M, Butenas S, Zmudka K, Mann KG (2008) Thrombin generation in acute coronary syndrome and stable coronary artery disease: dependence on plasma factor composition. J Thromb Haemost 6:104–110
Peters M, Plaat BE, Cate HT, Wolters HJ, Weening RS, Brandjes DP (1994) Enhanced thrombin generation in children with sickle cell disease. Thromb Haemost 71(2):169–172
Meddahi S, Bara L, Fessi H, Samama MM (2004) Standard measurement of clot-bound thrombin by using a chromogenic substrate for thrombin. Thromb Res 114(1):51–56
Aurell L, Friberger P, Karlsson G, Claeson G (1977) A new sensitive and highly specific chromogenic peptide substrate for factor Xa. Thromb Res 11:595–609
Bock LC, Griffin LC, Latham JA, Vermaas ER, Toole JJ (1992) Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature 355(6360):564–566
Wang XY, Dong P, Yun W, Xu Y, He PG, Fang YZ (2009) A solid-state electrochemiluminescence biosensing switch for detection of thrombin based on ferrocene-labeled molecular beacon aptamer. Biosens Bioelectron 24(11):3288–3292
Hansen JA, Wang J, Kawde AN, Xiang Y, Gothelf KV, Collins G (2006) Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor. J Am Chem Soc 128:2228–2229
Degefa TH, Hwang S, Kwon D, Park JH, Kwak J (2009) Aptamer-based electrochemical detection of protein using enzymatic silver deposition. Electrochim Acta 54(27):6788–6791
Ikebukuro K, Kiyohara C, Sode K (2005) Novel electrochemical sensor system for protein using the aptamers in sandwich manner. Biosens Bioelectron 20(10):2168–2172
Suprun E, Shumyantseva V, Bulko T, Rachmetova S, Rad’ko S, Bodoev N, Archakov A (2008) Au-nanoparticles as an electrochemical sensing platform for aptamer—thrombin interaction. Biosens Bioelectron 24(4):825–830
Zhang XR, Qi BP, Li Y, Zhang SS (2009) Amplified electrochemical aptasensor for thrombin based on bio-barcode method. Biosens Bioelectron 25(1):259–262
Wei H, Li BL, Li J, Wang EK, Dong SJ (2007) Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes. Chem Commun, pp 3735–3737
Li T, Wang EK, Dong SJ (2008) G-quadruplex-based DNAzyme for facile colorimetric detection of thrombin. Chem Commun, pp 3654–3656
Pavlov V, Xiao Y, Shlyahovsky B, Willner I (2004) Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. J Am Chem Soc 126(38):11768–11769
Wang XY, Zhou JM, Yun W, Xiao SS, Chang Z, He PG, Fang YZ (2007) Detection of thrombin using electrogenerated chemiluminescence based on Ru(bpy) 2+3 -doped silica nanoparticle aptasensor via target protein-induced strand displacement. Anal Chim Acta 598(2):242–248
Gill R, Polsky R, Willner I (2006) Pt nanoparticles functionalized with nucleic acid act as catalytic labels for the chemiluminescent detection of DNA and proteins. Small 2(8–9):1037–1041
Li T, Wang EK, Dong SJ (2008) Chemiluminescence thrombin aptasensor using high-activity DNAzyme as catalytic label. Chem Commun, pp 5520–5522
Wang WJ, Chen CL, Qian MX, Zhao XS (2008) Aptamer biosensor for protein detection using gold nanoparticles. Anal Biochem 373(2):213–219
Wang YL, Wei H, Li BL, Ren W, Guo SJ, Dong SJ, Wang EK (2007) SERS opens a new way in aptasensor for protein recognition with high sensitivity and selectivity. Chem Commun, pp 5220–5222
Bizzarri AR, Cannistraro S (2009) Surface-enhanced Raman spectroscopy combined with atomic force microscopy for ultrasensitive detection of thrombin. Anal Biochem 393(2):149–154
Liu SP, Luo HQ, Li NB, Liu ZF, Zheng WX (2001) Resonance rayleigh scattering study of the interactionof heparin with some basic diphenyl naphthylmethane dyes. Anal Chem 73:3907–3914
Luo HQ, Li NB, Liu SP (2006) Resonance rayleigh scattering study of interaction of hyaluronic acid with ethyl violet dye and its analytical application. Biosens Bioelectron 21:1186–1194
Liu ZD, Li J, Ling YF, Huang CZ (2009) A localized surface plasmon resonance light-scattering assay of mercury (II) on the basis of Hg2+-DNA complex induced aggregation of gold nanoparticles. Environ Sci Technol 43:5022–5027
Liang AH, Zhang NN, Jiang ZL, Wang SM (2008) A sensitive resonance scattering spectral assay for the determination of trace H2O2 based on the HRP catalytic reaction and nanogold aggregation. J Fluoresc 18:1035–1041
Jiang ZL, Zou MJ, Liang AH (2008) An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin. Clin Chim Acta 387:24–30
Liu KG, Yuan R, Chai YQ, Tang DP, An HZ (2010) [AuCl4]- and Fe3+/[Fe(CN)6]3- ions-derivated immunosensing interface for electrochemical immunoassay of carcinoembryonic antigen in human serum. Bioprocess Biosyst Eng 33:179–185
Jiang ZL, Liao XJ, Deng AP, Liang AH, Li JS, Pan HC, Li JF, Wang SM, Huang YJ (2008) Catalytic effect of nanogold on Cu(II)-N2H4 reaction and its application to resonance scattering immunoassay. Anal Chem 80:8681–8687
Wei XL, Zou MJ, Jiang ZL, Liu QY, Wen GQ (2008) A highly sensitive resonance scattering assay for immunoglubin M using Ag(I)-hydroquinone-immunonanogold catalytic reaction. Plasmonics 3:73–78
Jiang ZL, Fan YY, Chen ML, Liang AH, Liao XJ, Wen GQ, Shen XC, He XC, Pan HC, Jiang HS (2009) Resonance scattering spectral detection of trace Hg2+ using aptamer-modified nanogold as probe and nanocatalyst. Anal Chem 81:5439–5445
Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382(6592):607–609
Tasset DM, Kublik MF, Steiner W (1997) Oligonucleotide inhibitor of human thrombin that bind distinct epitopes. J Mol Biol 272(5):688–698
Spiridonova VA, Rog EV, Dugina TN, Strukova SM, Kopylov AM (2003) Aptamer DNA-a new type of thrombin inhibitor. Bioorg Khim 29(5):495–498
Jennifer AK, Juli F, Todd OY (1996) Reconciliation of the X-ray and NMR structures of the thrombin-binding aptamer d (GGTTGGTGTGGTTGG). J Mol Biol 256(3):417–422
Baldrich E, Restrepo A, O'Sullivan CK (2004) Aptasensor development: elucidation of critical parameters for optimal aptamer performance. Anal Chem 76:7053–7063
Nagatoishi S, Tanaka Y, Tsumoto K (2007) Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions. Biochem Biophys Res Commun 352(3):812–817
Tu YH, Cheng GF, Lin L, Zheng J, Wu ZR, He PG, Fang YZ (2006) Detection of thrombin with a novel nanobiosensor based on aptamer fluorescence. Chem J Chin Univ 27(12):2266–2270
Yang H, Ji J, Liu Y, Kong J, Liu B (2009) An aptamer-based biosensor for sensitive thrombin detection. Electrochem Commun 11(1):38–40
Song MY, Zhang YX, Li T, Wang ZX, Yin JF, Wang HL (2009) Highly sensitive detection of human thrombin in serum by affinity capillary electrophoresis/laser-induced fluorescence polarization using aptamers as probes. J Chromatogr A 1216(5):873–878
Zheng J, Feng WJ, Lin L, Zhang F, Cheng GF, He PG, Fang YZ (2007) A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. Biosens Bioelectron 23(3):341–347
Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 20667001, 20865002, 20965002), Natural Science Foundation of Guangxi (No.0991021Z).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liang, A., Li, J., Jiang, C. et al. Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe. Bioprocess Biosyst Eng 33, 1087–1094 (2010). https://doi.org/10.1007/s00449-010-0434-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-010-0434-6