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β-Galactosidase production by the psychrotolerant yeast Guehomyces pullulans 17-1 isolated from sea sediment in Antarctica and lactose hydrolysis

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Abstract

A psychrotolerant yeast Guehomyces pullulans 17-1 was isolated from sea sediment in Antarctica. It was found that it could yield both extracellular and cell-bound β-galactosidase. After optimization of the medium and cultivation conditions, it was found that the yeast strain produced over 17.2 U mL−1 of β-galactosidase activity within 120 h at the flask level while the yeast strain produced over 25.3 U mL−1 of β-galactosidase activity within 144 h during the 2-L fermentation. This is the highest β-galactosidase activity produced by the wild type yeast strains reported so far. However, the optimal pH and temperature for the crude β-galactosidase were 4.0 and 50 °C, respectively. Lactose could be converted into glucose and galactose and a large amount of reducing sugar could be released from milk under catalysis of the yeast culture.

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Acknowledgments

This work was supported by National Infrastructure of Natural Resources for Science 386 and Technology Program of China (No. 2005DKA21209).

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  1. Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, 266003, Shandong, China

    Chunli Song, Zhenming Chi, Jing Li & Xianghong Wang

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  1. Chunli Song
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  2. Zhenming Chi
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  3. Jing Li
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  4. Xianghong Wang
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Correspondence to Zhenming Chi.

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Song, C., Chi, Z., Li, J. et al. β-Galactosidase production by the psychrotolerant yeast Guehomyces pullulans 17-1 isolated from sea sediment in Antarctica and lactose hydrolysis. Bioprocess Biosyst Eng 33, 1025–1031 (2010). https://doi.org/10.1007/s00449-010-0427-5

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