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Chitooligosaccharides enzymatic production by Metarhizium anisopliae

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Abstract

The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL.

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Acknowledgments

The authors thank Financiadora de Estudos e Projetos (FINEP), Conselho Nacional de Desenvolvimentos Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for support that made this work possible.

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Authors and Affiliations

  1. Departamento De Engenharia Química, Centro De Tecnologia, Institute de Technologia, Av. Senador Salgado Filho, s/n, Lagoa Nova, 59072-970, Natal, Rio Grande do Norte, Brazil

    Cristiane Fernandes de Assis, Nathália Kelly Araújo, Márcia Regina da Silva Pedrini, Gorete Ribeiro de Macedo & Everaldo Silvino dos Santos

  2. Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, 59072-970, Natal, Rio Grande do Norte, Brazil

    Maria Giovana Binder Pagnoncelli

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  1. Cristiane Fernandes de Assis
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  2. Nathália Kelly Araújo
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Correspondence to Cristiane Fernandes de Assis.

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de Assis, C.F., Araújo, N.K., Pagnoncelli, M.G.B. et al. Chitooligosaccharides enzymatic production by Metarhizium anisopliae . Bioprocess Biosyst Eng 33, 893–899 (2010). https://doi.org/10.1007/s00449-010-0412-z

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  • DOI: https://doi.org/10.1007/s00449-010-0412-z

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