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Optimization of mono and diacylglycerols production from enzymatic glycerolysis in solvent-free systems

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Abstract

This work reports solvent-free enzymatic glycerolysis of olive oil with an immobilized lipase (Novozym 435) using Tween 40, Tween 65, Tween 80, Tween 85, Triton X-100, and soy lecithin as surfactants. The first step was the screening of two potential surfactants for Monoacylglycerol (MAG) and Diacylglycerol (DAG) production with a pre-established operating condition and 2 h of reaction time. Afterwards, a sequential experimental design strategy was carried out in order to optimize MAG and DAG production using Tween 65 and Triton X-100 as surfactants. The operating conditions that optimized MAG and DAG yields were 70 °C, stirring rate of 600 rpm, glycerol:olive oil molar ratio of 6:1, 16 wt% of surfactant Tween 65 and 9.0 wt% of Novozym 435, leading to a content of 26 and 17 wt% of MAG and DAG, respectively.

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Acknowledgments

The authors thank CAPES/PROCAD, CNPq and Intecnial Ltda for the financial support of this work and scholarships.

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Authors and Affiliations

  1. Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil

    Alexsandra Valério, Suzimara Rovani, Helen Treichel, Débora de Oliveira & J. Vladimir Oliveira

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  1. Alexsandra Valério
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  2. Suzimara Rovani
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  3. Helen Treichel
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  4. Débora de Oliveira
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  5. J. Vladimir Oliveira
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Correspondence to Helen Treichel.

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Valério, A., Rovani, S., Treichel, H. et al. Optimization of mono and diacylglycerols production from enzymatic glycerolysis in solvent-free systems. Bioprocess Biosyst Eng 33, 805–812 (2010). https://doi.org/10.1007/s00449-009-0402-1

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  • DOI: https://doi.org/10.1007/s00449-009-0402-1

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