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Construction of simultaneous SPR and QCM sensing platform

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Abstract

To construct a novel simultaneous SPR and QCM sensing system, an AT-cut quartz crystal is fabricated by sputtering 250 nm of ITO on one side of the quartz plate over a 5-nm thick underlay of titanium, while a 50-nm thick layer of gold is sputter-deposited on the other side to induce a total light reflection of an incident laser beam on the thin gold layer. The signals of the sensing system are detected using a Handy-SPR and QCA922 when dropping 200 μL of Milli-Q water into the sensing cell. A decrease in the SPR reflected light intensity is clearly identified. In the same experiment, the changes in the resonant frequency and resistance are about 2 kHz and 200 Ω, respectively. Furthermore, the oscillation stabilities of the resonant frequency and resistance are about 50 Hz and 2 Ω, respectively, which are sufficient to detect a large mass change on the QCM/SPR chip.

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Acknowledgment

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (KOSEF, 2009-0064245).

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Authors and Affiliations

  1. Department of Chemical Engineering, Dong-A University, 840 Hadan-Dong, Saha-Gu, Pusan, 604-714, Korea

    JongMin Kim, SeongHoon Kim & Sang-Mok Chang

  2. Department of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo, 192-0982, Japan

    Tatsuya Ohashi & Hiroshi Muramatsu

  3. Department of Chemical Engineering, ILRI, Kyunghee University, Seochun 1, Kiheung, Yongin, Kyungki-Do, 449-701, Korea

    Woo-Sik Kim

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  1. JongMin Kim
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  2. SeongHoon Kim
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  3. Tatsuya Ohashi
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  4. Hiroshi Muramatsu
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  5. Sang-Mok Chang
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Correspondence to Woo-Sik Kim.

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Kim, J., Kim, S., Ohashi, T. et al. Construction of simultaneous SPR and QCM sensing platform. Bioprocess Biosyst Eng 33, 39–45 (2010). https://doi.org/10.1007/s00449-009-0370-5

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  • DOI: https://doi.org/10.1007/s00449-009-0370-5

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